Hepatocyte growth factor/scatter factor activates the apoptosis signaling pathway by increasing caspase-3 activity in sarcoma 180 cells

Biochemical and Biophysical Research Communications
N ArakakiY Daikuhara

Abstract

Hepatocyte growth factor, which is now known to be the same protein as scatter factor, induced oligonucleosomal fragmentation of nuclear DNA of Sarcoma 180 cells and increased the activity of caspase-3, a key component in control of the apoptotic cell death pathway to about 2.6 times that in control cells on 48 hr incubation, but did not increase the activity of caspase-1. Both HGF-induced DNA fragmentation and caspase-3 activity were completely inhibited by co-incubation with an inhibitor of caspase-3, Ac-DEVD-H. In contrast, HGF did not affect the expression of the apoptosis suppressors Bcl-2 and Bcl-x. These results indicate that HGF activates the apoptosis signaling pathway by increasing caspase-3 activity in Sarcoma 180 cells.

References

Feb 1, 1992·Cell Biology International Reports·M MiyazakiI Yamamoto
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J S RubinP W Finch
Mar 15, 1991·Biochemical and Biophysical Research Communications·Y YoshiyamaH Tsubouchi
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K M WeidnerY Daikuhara
Nov 1, 1990·The Journal of Cell Biology·K M WeidnerW Birchmeier
Oct 21, 1994·Cell·Z N Oltvai, S J Korsmeyer
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S GiordanoP M Comoglio
Feb 21, 1997·Science·P Golstein

❮ Previous
Next ❯

Citations

Oct 6, 2007·Cell Death and Differentiation·D Tulasne, B Foveau
Nov 16, 2004·Molecular and Cellular Biology·David TulasneVéronique Fafeur
Mar 27, 2007·Annals of the New York Academy of Sciences·Catherine LeroyVéronique Fafeur
Sep 29, 2011·American Journal of Reproductive Immunology : AJRI·Ehsan SoleymaninejadianEsmaeil Samadian
May 30, 2006·Biochemical and Biophysical Research Communications·Shuli Xia, John Laterra
Feb 28, 2002·Molecular Cell·Xue WangReza Zarnegar
Apr 16, 2015·Stem Cells Translational Medicine·Tullia MaraldiAnto De Pol
Dec 7, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yunqing LiRoger Abounader
Jul 1, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H Tsubouchi
Mar 14, 2000·Cell Death and Differentiation·M L De SantisD S Salomon
Oct 23, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Naokatu ArakakiTomihiko Higuti
May 1, 1999·The Journal of Biological Chemistry·N ArakakiY Daikuhara
Jun 3, 2011·Growth Factors·Barbara Grzelakowska-Sztabert, Magdalena Dudkowska
Jun 17, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bachchu LalJohn Laterra
Apr 9, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Francine WalkerThérèse Lehy

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis