Hepatocyte growth factor/scatter factor binds with high affinity to dermatan sulfate.

The Journal of Biological Chemistry
M LyonJ T Gallagher

Abstract

We have demonstrated by affinity chromatography that hepatocyte growth factor/scatter factor (HGF/SF) binds strongly to dermatan sulfate (DS), with a similar ionic strength dependence to that previously seen with heparan sulfate (HS). Analysis of binding kinetics on a biosensor yields an equilibrium dissociation constant, KD, of 19.7 nM. This corresponds to a 10-100-fold weaker interaction than that with HS, primarily due to a faster dissociation rate of the complex. The smallest DS oligosaccharide with significant affinity for HGF/SF by affinity chromatography appears to be an octasaccharide. A sequence comprising unsulfated iduronate residues in combination with 4-O-sulfated N-acetylgalactosamine is sufficient for high affinity binding. The presence of 2-O-sulfation on the iduronate residues does not appear to be inhibitory. These observations concur with our previous suggestions, from analyses of HS binding (Lyon, M., Deakin, J. A., Mizuno, K., Nakamura, T., and Gallagher, J.T. (1994) J. Biol. Chem. 269, 11216-11223), that N-sulfation of hexosamines and 2-O-sulfation of iduronates are not absolute requirements for glycosaminoglycan binding to HGF/SF. This is the first described example of a high affinity interaction between ...Continue Reading

References

May 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·T SambandamS L Ekborg
Nov 1, 1995·The Biochemical Journal·K SvennevigS O Kolset
Dec 8, 1995·The Journal of Biological Chemistry·S AshikariK Kimata
Jul 14, 1995·The Journal of Biological Chemistry·T F ZioncheckR J Stack
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D S GrantE M Rosen
May 1, 1994·Biochemical Society Transactions·M Lyon, J T Gallagher
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·G VenkataramanR Sasisekharan
Apr 7, 1993·Biochimica Et Biophysica Acta·M GigliV Rizzo
Apr 1, 1996·Journal of Biochemistry·K Matsumoto, T Nakamura

❮ Previous
Next ❯

Citations

Dec 28, 1999·Journal of Molecular Recognition : JMR·D G Myszka
Sep 16, 2000·European Journal of Pharmacology·E M DenholmP J Silver
Apr 1, 2000·Biochimica Et Biophysica Acta·O Habuchi
Jan 9, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M Lyon, J T Gallagher
May 22, 2003·Paediatric Respiratory Reviews·Janis ShuteAndrew Bush
Sep 6, 2002·Glycobiology·Janet M Trowbridge, Richard L Gallo
Apr 24, 2002·American Journal of Respiratory Cell and Molecular Biology·Yasushi SakamakiToshikazu Nakamura
Apr 23, 2013·International Journal of Cell Biology·Maryse DeleheddeRomain R Vivès
Jan 8, 2004·Growth Factors·J A DaviesJ E Turnbull
May 27, 2006·Endothelium : Journal of Endothelial Cell Research·Marco Rusnati, Marco Presta
Oct 11, 2012·Journal of Pharmaceutical Sciences·Tomoko SugiuraYukio Kato
Jan 1, 2013·Journal of Bioscience and Bioengineering·Shintaro NakamuraHiroyuki Ijima
Oct 7, 2008·Biochimica Et Biophysica Acta·Masahiro AsadaToru Imamura
Jun 19, 2007·Experimental Neurology·Dámaso CrespoJames W Fawcett
Jun 5, 2010·Journal of Cellular Biochemistry·Errol WijelathMichael Sobel
Dec 3, 2014·Progress in Neurobiology·John W WrightJoseph W Harding
Jul 14, 2016·Chemical Reviews·Marco MendeStefan Bräse
Jun 11, 2014·Experimental Biology and Medicine·Ewa M KoźmaKrystyna Olczyk
Feb 22, 2002·Biomedical Chromatography : BMC·Wenjun MaoRobert J Linhardt
Feb 24, 2007·Journal of Biochemistry·Ryoichi SakiyamaToshikazu Nakamura
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Norma Marchesini, Yusuf A Hannun

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.