Hepatocyte nuclear factor 1A (HNF1A) as a possible tumor suppressor in pancreatic cancer

PloS One
Zhaofan LuoDonghui Li

Abstract

HNF1A (Hepatocyte nuclear factor 1 alpha) is a transcription factor that is known to regulate pancreatic differentiation and maintain homeostasis of endocrine pancreas. Recently, genome-wide association studies have implicated HNF1A as a susceptibility gene for pancreatic cancer. However, the functional significance and molecular mechanism of HNF1A in pancreatic carcinogenesis remains unclear. Using RT-PCR, Western blot and immunohistochemistry methods, we examined HNF1A gene expression in eight pancreatic carcinoma cell lines and in paired tumor and normal tissue samples from patients with resected pancreatic ductal adenocarcinoma. We knocked down the HNF1A gene expression in two cancer cell lines using three siRNA sequences. The impacts on cell proliferation, apoptosis, and cell cycle as well as the phosphorylation of Akt signaling transduction proteins were examined using ATP assay, flow cytometry and Western blot. HNF1A was expressed in three out of eight pancreatic adenocarcinoma cell lines and the level of HNF1A mRNA and protein expression was significantly lower in tumors than in normal adjacent tissues by both RT-PCR and Western Blot analyses. Immunohistochemistry revealed that the level of HNF1A expression was signific...Continue Reading

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Citations

Nov 10, 2015·Biochemical and Biophysical Research Communications·Eun Hee HanYoung-In Chi
Feb 10, 2018·Nature Communications·Alison P KleinLaufey T Amundadottir
Dec 13, 2018·Journal of the National Cancer Institute·Naomi WalshRachael Z Stolzenberg-Solomon
Oct 1, 2019·Current Molecular Pharmacology·Sumreen Begum
Nov 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lili GuShuhua Tan
Nov 21, 2020·Scientific Reports·Ramadevi SubramaniRajkumar Lakshmanaswamy
Oct 24, 2020·Clinical and Translational Gastroenterology·Federica IsidoriSandro Mattioli
Sep 14, 2019·Endocrine Regulations·Terezia ValkovicovaDaniela Gasperikova

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Methods Mentioned

BETA
protein extraction
electrophoresis
transfections
transfection
flow cytometry
FCS
PCR
Fluorescence-activated cell sorting
flow
gene knockdown

Software Mentioned

FCS Express

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