Hepatocyte nuclear factor-4alpha regulates human cellular retinol-binding protein type II gene expression in intestinal cells

American Journal of Physiology. Gastrointestinal and Liver Physiology
Noriaki YamaguchiKazuhito Suruga

Abstract

Cellular retinol-binding protein type II (CRBPII) is abundantly expressed in the small intestinal enterocytes of many vertebrates and plays important physiological roles in intestinal absorption, transport, and metabolism of vitamin A. In the present study, we investigated regulation of human CRBPII gene expression using human intestinal Caco-2 BBe cells. We found that the human CRBPII gene contained a direct repeat 1 (DR-1)-like nuclear receptor response element in the proximal promoter region and that endogenous hepatocyte nuclear factor-4alpha (HNF-4alpha) was a major transcription factor binding to the DR-1-like element. Cotransfection of HNF-4alpha expression vector transactivated the human CRBPII gene promoter activity, whereas mutation of the DR-1-like element abolished the promoter activity. Stably transfected Caco-2 BBe cells overexpressing HNF-4alpha significantly increased endogenous CRBPII gene expression and retinyl ester synthesis. Reduction of HNF-4alpha protein levels by HNF-4alpha small interference RNA decreased CRBPII gene expression. Caco-2 BBe cells treated with phorbol 12-myristate 13-acetate, a protein kinase C activator, decreased nuclear HNF-4alpha protein level and binding activity to the human CRBPII ...Continue Reading

References

Jun 15, 1992·Biochimica Et Biophysica Acta·S HataT Osumi
Dec 18, 1990·Biochemistry·T C Quick, D E Ong
Oct 1, 1987·Applied Biochemistry and Biotechnology·R J Linhardt
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J A Crow, D E Ong
Feb 1, 1995·The American Journal of Physiology·T W LissoosM S Levin
Nov 1, 1994·Molecular and Cellular Biology·E SuhP G Traber
Aug 26, 1998·Biochemical and Biophysical Research Communications·A DuringJ C Smith
May 23, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Liang ZhangEllen Li
Dec 31, 2002·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Ming PanWiley W Souba
Apr 16, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Line OlsenJorgen Olsen
May 14, 2005·Histochemistry and Cell Biology·Larry L SwiftDavid E Ong
Jul 14, 2005·Annual Review of Nutrition·Earl H Harrison
Oct 18, 2005·Nature Reviews. Molecular Cell Biology·Walter Kolch
Dec 3, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kwang-Hoon Song, John Y L Chiang
Dec 15, 2005·The British Journal of Nutrition·Kazuhito SurugaToshinao Goda
Mar 9, 2007·Biochemical and Biophysical Research Communications·Maja KlapperFrank Döring
Jun 9, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shuangying LengDennis D Black
Feb 20, 2008·Life Sciences·Noriaki Yamaguchi, Kazuhito Suruga
Feb 29, 2008·Drug Metabolism and Pharmacokinetics·Frank J Gonzalez

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Citations

May 5, 2016·The Journal of Nutritional Biochemistry·Sarah C CottinHarry J McArdle
Apr 15, 2014·Molecular Biology Reports·Yan WangQing Zhu
May 30, 2020·Critical Reviews in Biochemistry and Molecular Biology·William S BlanerMarcin Golczak

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