Hepatocyte nuclear factor 6 inhibits the growth and metastasis of cholangiocarcinoma cells by regulating miR-122

Journal of Cancer Research and Clinical Oncology
Huaqiang ZhuXueying Sun

Abstract

Hepatocyte nuclear factor 6 (HNF6) is a liver-enriched transcription factor and highly expressed in mature bile duct epithelial cells. This study sought to investigate the role of HNF6, particularly the molecular mechanisms for how HNF6 is involved in the growth and metastasis of cholangiocarcinoma (CCA) cells. The expression of HNF6, miR-122 and key molecules was examined by Western blot analysis and real-time RT-PCR. Stable transfectants, HCCC-HNF(low) and RBE-HNF(high), were generated from human CCA HCCC-9810 and RBE cells, respectively. The regulatory effect of HNF6 on miR-122 was evaluated by luciferase reporter assay. Cell proliferation, cycle distribution, migration and invasion were analyzed. The xenograft model was used to assess the effects of HNF6 overexpression on tumorigenesis, growth, metastasis and therapeutic potentials. Human CCA tissues and cells expressed lower levels of HNF6, which positively correlated with miR-122. HNF6 regulated the expression of miR-122 by stimulating its promoter. HNF6 overexpression inhibited cell proliferation by inducing cell cycle arrest at G1 phase through regulating miR-122, cyclin G1 and insulin-like growth factor-1 receptor. HNF6 inhibited the migration and invasion of CCA cells...Continue Reading

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Citations

Jun 16, 2017·Liver International : Official Journal of the International Association for the Study of the Liver·Huaqiang ZhuJun Lu
Sep 27, 2018·Journal of Cellular Physiology·Kai JiangKefeng Ding
Dec 3, 2020·Annual Review of Pathology·Pedro M RodriguesJesus M Banales
Apr 24, 2021·Expert Review of Gastroenterology & Hepatology·Antonio CiglianoDiego F Calvisi

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