PMID: 2121278Nov 9, 1990Paper

Hepatocyte specific long lasting inhibition of protein N-glycosylation by D-galactosamine

Biochimica Et Biophysica Acta
V GrossW Gerok

Abstract

The effect of D-galactosamine on protein N-glycosylation was studied in rat hepatocyte primary cultures for alpha 1-antitrypsin (three complex type oligosaccharide chains) and alpha 1-acid glycoprotein (six complex type oligosaccharide chains). D-Galactosamine at a concentration of 4 mM inhibited partially de novo N-glycosylation leading to the formation of alpha 1-antitrypsin lacking one to two and of alpha 1-acid glycoprotein lacking one to five of its carbohydrate side chains. In addition D-galactosamine interfered with oligosaccharide processing, leading to the formation of some carbohydrate side chains remaining in an endoglucosaminidase H sensitive, i.e., not completely processed, form. D-Galactosamine impaired the secretion of alpha 1-antitrypsin and of alpha 1-acid glycoprotein but did not inhibit the secretion of the unglycosylated albumin. The inhibitory effect of D-galactosamine on de novo glycosylation as well as on oligosaccharide processing lasted for at least 24 h after it had been removed from the cells. D-Galactosamine impaired the glycosylation of alpha 1-antitrypsin only in hepatocytes, but not in human monocytes. Furthermore, D-galactosamine did not impair the N- and O-glycosylation of interleukin-6 in human...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M L MaccecchiniG Schatz
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C GalanosW Reutter
May 14, 1979·Biochemical and Biophysical Research Communications·N R KatzP T Wilson
Feb 1, 1985·Experimental and Molecular Pathology·T A Tran-ThiK Decker
Sep 15, 1986·European Journal of Biochemistry·S Giffhorn-Katz, N R Katz
Feb 1, 1986·The Biochemical Journal·W McDowellR T Schwarz
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Oct 1, 1968·Experimental and Molecular Pathology·D KepplerK Decker
Dec 28, 1971·Journal of Molecular Biology·J King, U K Laemmli
Mar 1, 1984·Experimental Cell Research·V GrossP C Heinrich
Nov 1, 1982·European Journal of Biochemistry·G Weckbecker, D O Keppler
Feb 1, 1983·Proceedings of the Society for Experimental Biology and Medicine·D S CamaraJ P Nolan
Jul 15, 1984·Biochemical Pharmacology·G Weckbecker, D O Keppler
Jul 1, 1983·European Journal of Biochemistry·T AndusP C Heinrich
Jan 1, 1981·Annual Review of Biochemistry·S C Hubbard, R J Ivatt
Oct 1, 1981·Archives of Biochemistry and Biophysics·J A Hanover, W J Lennarz
Jun 1, 1980·Experimental and Molecular Pathology·J RasenackK Decker
May 1, 1968·The Biochemical Journal·F MaleyR Delgiacco
Feb 14, 2007·Chemistry : a European Journal·Dieter EndersAudrey Herrbach

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