Hepatocyte spheroid formation on a titanium dioxide gel surface and hepatocyte long-term culture.

Journal of Materials Science. Materials in Medicine
Kohji NakazawaT Kunitake

Abstract

The cell morphology and expression of differentiated functions of primary rat hepatocytes on a titanium dioxide (TiO(2)) gel surface were investigated. Polystyrene culture dishes were coated with TiO(2) gel by spin-coating an ethanol solution of titanium n-butoxide, hydrolyzing in a humidity chamber and drying with nitrogen gas. The TiO(2) gel layer formed on the polystyrene dishes was transparent and robust, and its surface was quite flat. Rat hepatocytes inoculated on the TiO(2) gel-coated polystyrene dishes gradually accumulated with increasing culture time, and then spontaneously formed many hepatocyte spheroids at 90 +/- 21 microm diameter from about 3 days of culture. The diameter of the spheroids increased during the culture, and was 151 +/- 43 microm at 14 days of culture. Ammonia removal and albumin secretion by hepatocytes on the TiO(2) gel-coated polystyrene dishes were maintained at a high level for at least 14 days of culture compared with on a type I collagen-coated dish and a non-coated polystyrene dish. These results indicate that TiO(2) gel is an adequate material for hepatocyte spheroid formation and long-term culture of spheroids.

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Citations

Aug 9, 2007·Biomedical Microdevices·Mimi Y ZhangMohammed R K Mofrad
Mar 13, 2014·International Journal of Molecular Sciences·Shimaa A AbdellatefAkiyoshi Taniguchi
Jul 31, 2013·Journal of Bioscience and Bioengineering·Ryosuke TakahashiAkiko Hisada
Mar 9, 2011·Biochemical and Biophysical Research Communications·Mahboobe GhaediAlexander Revzin
Apr 21, 2009·Biomaterials·Caroline N JonesAlexander Revzin
May 17, 2008·Journal of Biomedical Materials Research. Part a·Mónica Carballo-VilaJorge E Collazos-Castro
Mar 21, 2009·Journal of Biomedical Materials Research. Part a·John D JarrellJeffrey R Morgan
Oct 23, 2012·Journal of Tissue Engineering and Regenerative Medicine·Mahboobe GhaediAlexander Revzin
Mar 19, 2013·Biochemical and Biophysical Research Communications·Derek Yip, Cheul H Cho
Jun 15, 2007·Journal of Biomedical Materials Research. Part a·John D JarrellJeffrey R Morgan
Jun 1, 2012·Macromolecular Bioscience·Ashleigh CooperMiqin Zhang

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