Hepatocyte transplantation in bile salt export pump-deficient mice: selective growth advantage of donor hepatocytes under bile acid stress.

Journal of Cellular and Molecular Medicine
Huey-Ling ChenMei-Hwei Chang

Abstract

The bile salt export pump (Bsep) mediates the hepatic excretion of bile acids, and its deficiency causes progressive familial intrahepatic cholestasis. The current study aimed to induce bile acid stress in Bsep(-/-) mice and to test the efficacy of hepatocyte transplantation in this disease model. We fed Bsep(-/-) and wild-type mice cholic acid (CA) or ursodeoxycholic acid (UDCA). Both CA and UDCA caused cholestasis and apoptosis in the Bsep(-/-) mouse liver. Wild-type mice had minimal liver injury and apoptosis when fed CA or UDCA, yet had increased proliferative activity. On the basis of the differential cytotoxicity of bile acids on the livers of wild-type and Bsep(-/-) mice, we transplanted wild-type hepatocytes into the liver of Bsep(-/-) mice fed CA or CA + UDCA. After 1-6 weeks, the donor cell repopulation and canalicular Bsep distribution were documented. An improved repopulation efficiency in the CA + UDCA-supplemented group was found at 2 weeks (4.76 ± 5.93% vs. 1.32 ± 1.48%, P = 0.0026) and at 4-6 weeks (12.09 ± 14.67% vs. 1.55 ± 1.28%, P < 0.001) compared with the CA-supplemented group. Normal-appearing hepatocytes with prominent nuclear staining for FXR were noted in the repopulated donor nodules. After hepatocyte ...Continue Reading

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Citations

Apr 7, 2016·Hepatology International·Liyanne F M van de LaarschotSteven W M Olde Damink
Nov 29, 2017·Cellular and Molecular Life Sciences : CMLS·Salamah M AlwahshDavid C Hay
Oct 28, 2018·Journal of Biomedical Science·Huey-Ling ChenMei-Hwei Chang
Dec 20, 2019·Stem Cells and Development·Tahera AnsariPaul D Sibbons
Jan 2, 2021·International Journal of Molecular Sciences·Piter J BosmaRonald Pj Oude-Elferink

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Methods Mentioned

BETA
PCR
genotyping

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis