Hepatoprotective activity of thymoquinone in isolated rat hepatocytes

Toxicology Letters
Mohamad-Hesham Y Daba, M S Abdel-Rahman

Abstract

Thymoquinone, the active constituent of Nigella sativa, was tested in isolated rat hepatocytes as a hepatoprotective agent against tert-butyl hydroperoxide (TBHP) toxicity. TBHP (2 mM) was used to produce oxidative injury in isolated rat hepatocytes and caused progressive depletion of intracellular glutathione (GSH), loss of cell viability as evidenced by trypan blue uptake and leakage of cytosolic enzymes, alanine transaminase (ALT) and aspartic transaminase (AST). Preincubation of hepatocytes with 1 mM of either thymoquinone or silybin, which is a known hepatoprotective agent, resulted in the protection of isolated hepatocytes against TBHP induced toxicity evidenced by decreased leakage of ALT and AST, and by decreased trypan blue uptake in comparison to TBHP treated hepatocytes. Both thymoquinone and silybin prevented TBHP induced depletion of GSH to the same extent. Although thymoquinone protected the liver enzymes leakage, the degree of protection was less than that caused by silybin.

References

Jun 1, 1975·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H BaurE Pfaff
Feb 1, 1992·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·E BosisioO Pirola
Apr 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G J GoresJ J Lemasters
Mar 1, 1989·Archives of Biochemistry and Biophysics·N MasakiJ L Farber
Apr 1, 1983·Journal of Toxicology and Environmental Health·D L StoryD H Gould
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G BellomoS Orrenius
Apr 1, 1994·Chemico-biological Interactions·M P MiguezK O Lindros
Aug 1, 1993·Planta medica·M Concepción NavarroM Pilar Utrilla

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Citations

Jan 25, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·Hossein HosseinzadehMarjan Nassiri-Asl
Aug 23, 2012·Cardiovascular Toxicology·Adel ShabanaHassan Al Thani
Jan 15, 2014·International Journal of Biological Macromolecules·Masood A KhanHina Younus
Jan 20, 2004·International Journal of Cardiology·Saleh C El-SalehMaha I Al-Khalaf
Jul 8, 2000·International Journal of Immunopharmacology·M L Salem, M S Hossain
May 17, 2003·Toxicology Letters·Afaf A Mahgoub
Feb 11, 2003·Journal of Ethnopharmacology·S A Al-Jishi, B Abuo Hozaifa
Jul 25, 2000·Journal of Ethnopharmacology·N M AbuharfeilS Von Kleist
Jun 6, 2002·British Poultry Science·A Al-HomidanS E I Adam
Aug 10, 2010·Critical Reviews in Food Science and Nutrition·Masood Sadiq Butt, Muhammad Tauseef Sultan
Sep 9, 2008·World Journal of Gastroenterology : WJG·Fahrettin YildizMuharrem Bitiren
Dec 19, 2012·Indian Journal of Pharmacology·Anwar S HassanSawsan S Al-Haroon
Jul 2, 2009·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Mohammad Tariq
Jun 2, 2015·Pakistan Journal of Biological Sciences : PJBS·Wessam Mohamad Abdel-Wahab
Jun 19, 2014·Canadian Journal of Physiology and Pharmacology·Ghada M Suddek
May 29, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Claudia-Crina TomaAndrei Mocan
Nov 12, 2009·Life Sciences·Leelavinothan Pari, Chandrasekaran Sankaranarayanan
Dec 9, 2008·Journal of Ethnopharmacology·Bouchra MeddahPierre Selim Haddad
Nov 18, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M KhaderP M Eckl
Feb 13, 2007·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Hossein HosseinzadehToktam Ziaee
Nov 5, 2004·The Journal of Pharmacy and Pharmacology·Jing GaoQiang Xu
Oct 22, 2003·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·M KanterI Uygan
Dec 18, 2008·Phytotherapy Research : PTR·Khaled RadadWolf-Dieter Rausch
Feb 11, 2011·Drug Testing and Analysis·Camilia G MichelTaha S El-Alfy
Sep 20, 2005·The Journal of Nutritional Biochemistry·Xinhui TangZhong Ming Qian
Sep 26, 2008·Basic & Clinical Pharmacology & Toxicology·Abdel-Motaal M FoudaOsama A Sharaf El-Din
Sep 20, 2008·Basic & Clinical Pharmacology & Toxicology·Uz EbruErdemli Kemal
Apr 14, 2016·Inflammopharmacology·M Akram Khan, M Afzal
Dec 19, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Lizhi XuZhong-Ming Qian
Apr 2, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sara DarakhshanSajjad Sisakhtnezhad
Feb 26, 2013·Journal of Acupuncture and Meridian Studies·Hossein HosseinzadehElahe Taghiabadi

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