PMID: 8592682Nov 1, 1995Paper

Hepatoprotective activity of xanthones and xanthonolignoids against tert-butylhydroperoxide-induced toxicity in isolated rat hepatocytes--comparison with silybin

Pharmaceutical Research
E R FernandesO R Gottlieb

Abstract

Synthesize and evaluate the protective activity against tertbutylhydroperoxide-induced toxicity in freshly isolated rat hepatocytes of trans-kielcorin, trans-isokielcorin B, as well as their respective building blocks 3,4-dihydroxy-2-methoxyxanthone and 2,3-dihydroxy-4-methoxyxanthone. Wistar rats, weighing 200-250g were used. Hepatocyte isolation was performed by collagenase perfusion. Incubations were performed at 37 degrees C, using 1 million cells per milliliter in modified Krebs--Henseleit buffer. The protective activity was evaluated by measuring reduced and oxidized glutathione, lipid peroxidation and cell viability after inducing toxicity with tert-butylhydroperoxide (1.0 mM, 30 min), with or without the studied compounds in the concentrations of 0.025, 0.050, 0.100 and 0.200 mM. Silybin was tested in the same experimental conditions to serve as a positive control. Using these concentrations, the tested compounds prevented tert-butylhydroperoxide-induced lipid peroxidation and cell death in freshly isolated rat hepatocytes. All compounds were also effective in preventing perturbation of cell glutathione homeostasis in some extent. 3,4-Dihydroxy-2-methoxyxanthone and 2,3-dihydroxy-4-methoxyxanthone were more effective th...Continue Reading

Citations

Apr 13, 2005·Journal of Drug Targeting·Maribel TeixeiraMadalena Pinto
Jan 1, 2008·Acta Crystallographica. Section E, Structure Reports Online·Peizhong YuLiqing Chen
Mar 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carla FernandesMadalena M M Pinto
Jan 25, 2017·Journal of Food Science·Huijuan ZhangBaoguo Sun
Mar 16, 2001·In Vitro Cellular & Developmental Biology. Animal·F RemiãoM L Bastos
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Madalena M M PintoSara Cravo

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