Hepatoprotective Effect of Pericarpium zanthoxyli Extract Is Mediated via Antagonism of Oxidative Stress

Evidence-based Complementary and Alternative Medicine : ECAM
Sang Mi ParkRongjie Zhao

Abstract

Pericarpium zanthoxyli has been extensively used in traditional Oriental medicine to treat gastric disorders and has anti-inflammatory and antioxidative activities. Therefore, the present study examined a possible hepatoprotective effect of a P. zanthoxyli extract (PZE) and investigated the underlying molecular mechanisms. We employed an in vitro model of arachidonic acid (AA) + iron-induced hepatocyte damage and an in vivo model of CCl4-induced liver injury to assess the effects of PZE and evaluated the relevant molecular targets using biochemical assays, flow cytometry analysis, Western blot, and histopathological analysis. The PZE inhibited AA + iron-induced hepatotoxicity in HepG2 cells, improved mitochondrial dysfunction, and reversed an increase in the cellular H2O2 production and a decrease in the reduced GSH levels induced by AA + iron. Treatment with either 30 or 100 μg/ml PZE significantly increased the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) protein, and the latter dose also increased the antioxidant response element- (ARE-) driven luciferase activity and enhanced the protein expressions of glutamate-cysteine ligase catalytic subunit and NAD(P)H:quinone oxidoreductase 1. In addition, treatmen...Continue Reading

References

Jan 1, 1989·Pharmacology & Therapeutics·R O RecknagelR L Waller
Jun 1, 1995·Journal of Hepatology·K IshakR N MacSween
Sep 1, 1996·Free Radical Research·B Tadolini, G Hakim
Mar 1, 1997·Clinical and Experimental Pharmacology & Physiology·J Ludbrook
Aug 25, 2001·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·K Wellington, B Jarvis
Mar 27, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Laurie M Zipper, R Timothy Mulcahy
Apr 28, 2004·Free Radical Biology & Medicine·Anil K Jaiswal
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Rebecca Taub
Oct 8, 2004·Journal of Biochemistry and Molecular Biology·Jong-Min Lee, Jeffrey A Johnson
Nov 3, 2004·Trends in Molecular Medicine·Hozumi Motohashi, Masayuki Yamamoto
May 3, 2006·Biochimica Et Biophysica Acta·María A Balboa, Jesús Balsinde
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Apr 28, 2007·Free Radical Biology & Medicine·Shunsuke TanigawaDe-Xing Hou
Sep 1, 2007·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Mary K ManibusanDavid A Eastmond
Jul 26, 2008·European Journal of Pharmacology·Adriana Salazar-MontesJuan Armendáriz-Borunda
Apr 18, 2009·Toxicology and Applied Pharmacology·Scott A ReismanCurtis D Klaassen
Mar 1, 2011·Chemico-biological Interactions·Rebecca L SmathersDennis R Petersen
Mar 20, 2013·BMC Complementary and Alternative Medicine·Guang-Zhi DongYoung Woo Kim
May 30, 2013·Journal of Ginseng Research·Sung Hwan KiIl Je Cho
Jun 15, 2013·Oxidative Medicine and Cellular Longevity·Sang Mi ShinSung Hwan Ki
Jun 28, 2014·European Journal of Pharmacology·Guang-Zhi DongYoung Woo Kim
Jun 10, 2016·Nature Reviews. Disease Primers·Vicente ArroyoBernd Schnabl
Jun 25, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Qiang ZhaoCheng-Hai Liu
May 6, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Ji Yun JungSang Chan Kim

❮ Previous
Next ❯

Citations

Apr 20, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Chul Won LeeWon G An

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
FACS
Transfection
pharmacotherapy
nuclear translocation
nuclear

Software Mentioned

i Solution FL
SPSS
Empower
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.