Hepatotoxic potential of asarones: in vitro evaluation of hepatotoxicity and quantitative determination in herbal products

Frontiers in Pharmacology
Dhavalkumar N PatelHwee-Ling Koh

Abstract

α and β asarones are natural constituents of some aromatic plants, especially species of the genus Acorus (Araceae). In addition to reports of beneficial properties of asarones, genotoxicity and carcinogenicity are also reported. Due to potential toxic effects of β-asarone, a limit of exposure from herbal products of ~2 μg/kg body weight/day has been set temporarily until a full benefit/risk assessment has been carried out by the European Medicines Agency. Therefore, it is important to monitor levels of β-asarone in herbal products. In this study, we developed a simple, rapid and validated GC-MS method for quantitative determination of asarones and applied it in 20 pediatric herbal products after detecting high concentrations of β-asarone in a product suspected to be implicated in hepatotoxicity in a 3 month old infant. Furthermore, targeted toxicological effects were further investigated in human hepatocytes (THLE-2 cells) by employing various in vitro assays, with the goal of elucidating possible mechanisms for the observed toxicity. Results showed that some of the products contained as much as 4-25 times greater amounts of β-asarone than the recommended levels. In 4 of 10 samples found to contain asarones, the presence of as...Continue Reading

References

Nov 4, 1989·BMJ : British Medical Journal·F B MacGregorP C Hayes
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A M PfeiferB F Trump
Mar 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G Hasheminejad, J Caldwell
Apr 1, 1993·Planta medica·M L LópezT Mendoza-Figueroa
Jan 1, 1997·Journal of Ethnopharmacology·L GarduñoG A Chamorro
Dec 24, 1998·Journal of Pharmaceutical and Biomedical Analysis·R OpreanL Roman
Nov 24, 1999·Free Radical Biology & Medicine·H Sies
Nov 24, 1999·Free Radical Biology & Medicine·O W Griffith
May 23, 2002·Seminars in Liver Disease·Catherine Stedman
Feb 21, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kristine L WillettLarry Walker
Jan 22, 2005·Carcinogenesis·Rafael MayoralPaloma Martín-Sanz
Jul 23, 2005·Free Radical Research·Pietro Ghezzi
Sep 21, 2005·Journal of Hepatology·Felix StickelDetlef Schuppan
Nov 19, 2008·Fibrogenesis & Tissue Repair·Erica Novo, Maurizio Parola
Dec 17, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M TripathiP Kakkar
Jan 27, 2009·Biological Chemistry·Nazzareno BallatoriChristine L Hammond
Jun 10, 2009·Planta medica·Chu ChenBeat Meier
Oct 15, 2009·Seminars in Liver Disease·Victor J Navarro
Dec 31, 2009·Journal of Analytical Toxicology·Kristian BjörnstadOlof Beck
Mar 24, 2010·Toxicology Letters·Sudipta SahaEric Chun Yong Chan
May 13, 2010·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Jicheng LiuYingcai Niu
Jul 27, 2010·Bioorganic & Medicinal Chemistry Letters·Nigel GreeneYvonne Will
Nov 16, 2010·Chemico-biological Interactions·Richard A ThompsonJ Gerry Kenna
Mar 29, 2011·Mutation Research·Divyasree Sandeep, Cherupally Krishnan Krishnan Nair
Dec 3, 2011·British Journal of Cancer·J W ZimmermanB Pasche
Feb 22, 2012·European Journal of Drug Metabolism and Pharmacokinetics·Yong Qi FangRuo Ming Fang
Jun 29, 2012·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Dhavalkumar Narendrabhai PatelHwee-Ling Koh
Jul 27, 2012·British Journal of Clinical Pharmacology·Rolf TeschkeAxel Eickhoff
Sep 12, 2012·Forensic Science International : Synergy·Dariusz Zuba, Bogumiła Byrska
Sep 26, 2012·Scientia pharmaceutica·Pascal Unger, Matthias F Melzig
Oct 16, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Violetta Krajka-KuźniakWanda Baer-Dubowska
Sep 12, 2013·Journal of Pharmacy & Pharmaceutical Sciences : a Publication of the Canadian Society for Pharmaceutical Sciences, Société Canadienne Des Sciences Pharmaceutiques·Reem J Abdualmjid, Consolato Sergi

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Methods Mentioned

BETA
electrophoresis
MDA

Software Mentioned

Calcusyn

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