Hepcidin expression in iron overload diseases is variably modulated by circulating factors.

PloS One
Giulia RavasiAlberto Piperno

Abstract

Hepcidin is a regulatory hormone that plays a major role in controlling body iron homeostasis. Circulating factors (holotransferrin, cytokines, erythroid regulators) might variably contribute to hepcidin modulation in different pathological conditions. There are few studies analysing the relationship between hepcidin transcript and related protein expression profiles in humans. Our aims were: a. to measure hepcidin expression at either hepatic, serum and urinary level in three paradigmatic iron overload conditions (hemochromatosis, thalassemia and dysmetabolic iron overload syndrome) and in controls; b. to measure mRNA hepcidin expression in two different hepatic cell lines (HepG2 and Huh-7) exposed to patients and controls sera to assess whether circulating factors could influence hepcidin transcription in different pathological conditions. Our findings suggest that hepcidin assays reflect hepatic hepcidin production, but also indicate that correlation is not ideal, likely due to methodological limits and to several post-trascriptional events. In vitro study showed that THAL sera down-regulated, HFE-HH and C-NAFLD sera up-regulated hepcidin synthesis. HAMP mRNA expression in Huh-7 cells exposed to sera form C-Donors, HFE-HH an...Continue Reading

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Citations

Nov 17, 2012·Annals of Hematology·Rekha AthiyarathEunice Sindhuvi Edison
Apr 5, 2013·Biotechnology Letters·Yaghoub YazdaniAbbas Rezaei
Jul 30, 2014·Blood Cells, Molecules & Diseases·Giulia RavasiAlberto Piperno
Nov 5, 2013·Clinical & Developmental Immunology·Fernanda G VersianiPaulo A Nogueira
Sep 3, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Paul C Adams
Feb 9, 2013·The Biochemical Journal·Edina PandurZsuzsanna Fekete
Aug 14, 2015·PloS One·Aggeliki LyberopoulouGeorge Simos
Mar 31, 2015·Gene·Azza Aboul-EneinHala Abd El-Samie
Jun 23, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Sandra MilicSmiljana Ristic
Dec 14, 2019·Annals of Hematology·Rasha Abd El-Rahman El-GamalShaimaa Abdelmalik Pessar
Jun 3, 2021·Nutrients·Aoibhín Moore HeslinBreige A McNulty

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Methods Mentioned

BETA
genotyping
biopsy
ELISA
PCR

Software Mentioned

GraphPad
GRAPHPAD PRISM

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