Hepcidin Mediates Transcriptional Changes in Ferroportin mRNA in Differentiated Neuronal-Like PC12 Cells Subjected to Iron Challenge

Molecular Neurobiology
Steinunn Sara HelgudottirTorben Moos

Abstract

Ferroportin is the only known iron exporter, and its regulation seems to be controlled at both transcriptional, post-transcriptional, and post-translational levels. The objective of the current work was to investigate how cellular iron status affects the expression of the ferroportin gene Fpn under the influence of hepcidin, known to post-translational lower the available ferroportin protein. Nerve growth factor-beta (NGF-β)-differentiated PC12 cells, used as a model of neuronal cells, were evaluated in terms of their viability and expression of ferroportin after inducing cellular iron overload with ferric ammonium citrate (FAC) or hepcidin, iron deficiency with deferoxamine (DFO), or hepcidin in combination with FAC or DFO. Ferritin mRNA was significantly upregulated following treatment with 20 mM FAC. The viability of the differentiated PC12 cells was significantly reduced after treatment with 30 mM FAC or 1.0 μM hepcidin, but when combining FAC and hepcidin treatment, the cells remained unaffected. The expression of Fpn was concurrently upregulated after treatment with FAC in combination with hepcidin. Fifty millimolar DFO also increased Fpn. Together, these data point towards a transcriptional induction of Fpn in response t...Continue Reading

References

Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jayong ChungMarianne Wessling-Resnick
Aug 13, 2005·Neurochemistry International·Yanmin ChenYa Ke
Apr 10, 2007·Nature Structural & Molecular Biology·Mayka SanchezMatthias W Hentze
Jul 14, 2007·The Journal of Biological Chemistry·Hailing Yang, Fernando Cabral
Oct 24, 2007·Journal of Neurochemistry·Torben MoosEvan H Morgan
Jan 15, 2009·The Journal of Nutrition·Fikret AydemirMitchell D Knutson
Jun 10, 2010·The Journal of Clinical Investigation·Ivana De DomenicoJerry Kaplan
Jul 7, 2010·Cell·Matthias W HentzeClara Camaschella
Sep 3, 2010·Journal of Neural Transmission·Robert R CrichtonRoberta J Ward
Oct 22, 2011·Gut·Rayko Evstatiev, Christoph Gasche
Mar 24, 2012·Biochimica Et Biophysica Acta·Diane M Ward, Jerry Kaplan
Apr 14, 2012·Nature Reviews. Drug Discovery·Cheryl H ArrowsmithMatthieu Schapira
Aug 25, 2012·The Journal of Biological Chemistry·Farah H BardaiSantosh R D'Mello
Oct 25, 2013·Nature·Rahul M Kohli, Yi Zhang
Nov 13, 2013·Cellular and Molecular Life Sciences : CMLS·Hjalte Holm AndersenTorben Moos
Nov 21, 2013·Acta Neuropathologica Communications·Animesh Alexander RahaRuma Raha-Chowdhury
Nov 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Erik R AndersonYatrik M Shah
Apr 29, 2014·The FEBS Journal·Maria C Bonaccorsi di PattiGiovanni Musci
Jul 6, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sukru GulecJames F Collins
Jul 23, 2014·Free Radical Biology & Medicine·Darius J R Lane, Des R Richardson
Aug 15, 2014·Experimental and Therapeutic Medicine·Guo-Yang ZhaoYou-Jia Xu
Sep 11, 2014·Pharmacology & Therapeutics·Luc RochetteCatherine Vergely
Sep 19, 2014·Lancet Neurology·Roberta J WardLuigi Zecca
Apr 7, 2015·Biochimica Et Biophysica Acta·Bruno Silva, Paula Faustino
May 23, 2015·Nature Reviews. Drug Discovery·Moshe Szyf
Jul 24, 2015·Acta Biochimica Et Biophysica Sinica·Wenli GuoSijin Liu
Aug 4, 2015·Brain Research·Irfan A Qureshi, Mark F Mehler
Oct 7, 2015·Cell Metabolism·Hal DrakesmithTomas Ganz
Nov 8, 2015·Journal of Neurochemistry·Abdel A Belaidi, Ashley I Bush
Jun 24, 2016·Nature Reviews. Neuroscience·Bing YaoHongjun Song
Jul 23, 2016·Frontiers in Pharmacology·Giada SebastianiKostas Pantopoulos
Jan 28, 2017·Cell·Martina U MuckenthalerBruno Galy

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Citations

Jun 14, 2019·Biological Reviews of the Cambridge Philosophical Society·Zhong-Ming Qian, Ya Ke
Jun 17, 2020·Molecular Neurobiology·Steinunn Sara HelgudottirTorben Moos

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Methods Mentioned

BETA
FCS
PCR

Software Mentioned

Magellan2

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