Hepcidin messenger RNA expression in human lymphocytes.

Immunology
Jorge P PintoM de Sousa

Abstract

Hepcidin regulates intracellular iron levels by interacting with and promoting the degradation of ferroportin, a membrane protein and the only known cellular iron exporter. Studies of hepcidin expression and regulation have focused on its effects in innate immunity and as a regulator of systemic iron metabolism. In the present study we characterized the expression of hepcidin messenger RNA (mRNA) in human peripheral blood mononuclear cells (PBMCs) with a focus on peripheral blood lymphocytes (PBLs). We found that (1) all human PBMCs analyzed express basal hepcidin mRNA levels; (2) hepcidin mRNA expression increases after T-lymphocyte activation; (3) expression by PBLs increases in response to challenge by holotransferrin (Fe-TF) and by ferric citrate in vitro; (4) the Fe-TF-mediated up-regulation of hepcidin decreases ferroportin expression at the cytoplasmic membrane of PBLs; and (5) silencing of tumour necrosis factor-alpha (TNF-alpha) abrogates the effect of Fe-TF. In summary, we show that hepcidin expression determines intracellular iron levels by regulating the expression of ferroportin, as described in other cells, and that inappropriately low expression of hepcidin impairs normal lymphocyte proliferation. The results est...Continue Reading

References

Oct 1, 1974·The Journal of Experimental Medicine·J L Van SnickJ F Heremans
Aug 29, 1980·Science·M H DörnerM de Sousa
Feb 1, 1994·Immunology Letters·M de SousaG Porto
Feb 24, 1999·Journal of Gastroenterology and Hepatology·G J Anderson
May 3, 2000·The Journal of Clinical Investigation·J E LevyN C Andrews
Dec 7, 2000·The American Journal of Pathology·M M SantosM W Schilham
Feb 13, 2001·European Journal of Clinical Investigation·E M CardosoR Hultcrantz
May 31, 2001·British Journal of Haematology·L SahlstedtT Ruutu
Oct 24, 2002·Blood·Elsa M CardosoMaria de Sousa
Dec 17, 2002·Molecular and Cellular Biology·Pauline P WardOrla M Conneely
Mar 28, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Marco ConstanteManuela M Santos
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jaroslav TruksaErnest Beutler
Jul 20, 2006·Annual Review of Nutrition·Elizabeta Nemeth, Tomas Ganz
Aug 31, 2007·World Journal of Gastroenterology : WJG·Graça Porto, Maria De Sousa
Aug 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lijian WangBobby J Cherayil
Feb 24, 2009·Current Opinion in Immunology·Tomas Ganz
Apr 30, 2009·Annual Review of Pathology·Pauline L Lee, Ernest Beutler
Aug 15, 2009·Science·Chiara VecchiAntonello Pietrangelo

❮ Previous
Next ❯

Citations

Jul 28, 2012·Zeitschrift für Rheumatologie·M Wahle
May 17, 2014·Cellular and Molecular Life Sciences : CMLS·Diego Di LorenzoIsabella Zanella
Aug 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Andrew E ArmitagePersephone Borrow
Dec 20, 2011·Blood Cells, Molecules & Diseases·Liliana MarquesLuciana Costa
Feb 16, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·John D RyanJohn Crowe
Apr 26, 2012·European Journal of Immunology·Rachid BouchermaFrançois Huetz
Oct 23, 2010·Cellular Microbiology·Manfred NairzGünter Weiss
Jun 26, 2014·Transplant International : Official Journal of the European Society for Organ Transplantation·Benedikt SchaeferHeinz Zoller
Apr 26, 2011·Diabetes Research and Clinical Practice·Fang JiangXiao-Yang Jiao
Apr 2, 2011·Clinical Lymphoma, Myeloma & Leukemia·Bryan T CiccarelliSteven P Treon
Feb 4, 2014·Cancer Letters·Oriana MarquesCarlos Lopes
Mar 31, 2015·Gene·Azza Aboul-EneinHala Abd El-Samie
Dec 25, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Maria de Sousa
May 18, 2016·International Journal of Molecular Sciences·Ying-Hsien HuangHsing-Chun Kuo
Sep 20, 2011·Innate Immunity·José P Oliveira-FilhoAlexandre S Borges
Nov 15, 2013·Advances in Nutrition·Maria Carlota Dao, Simin Nikbin Meydani
Mar 4, 2020·FASEB BioAdvances·Xiyong FanAnthony M George
Nov 23, 2017·Frontiers in Immunology·Javier Arranz-TrullénEster Boix
Sep 21, 2018·Frontiers in Cell and Developmental Biology·Macha Samba-MondongaManuela M Santos
Aug 14, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·João V NevesPedro N S Rodrigues
Mar 23, 2021·Chemical Research in Toxicology·Glen ReidAnthony M George
Jun 4, 2018·International Journal of Pediatric Otorhinolaryngology·Umut GaziNerin Onder Bahceciler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.