PMID: 11323105Apr 27, 2001Paper

HEPES prevents edema in rat brain slices

Neuroscience Letters
D G MacGregorM E Rice

Abstract

Brain slices gain water when maintained in bicarbonate-buffered artificial cerebro-spinal fluid (ACSF) at 35 degrees C. We previously showed that this edema is linked to glutamate receptor activation and oxidative stress. An additional factor that may contribute to swelling is acidosis, which arises from high CO2 tension in brain slices. To examine the role of acidosis in slice edema, we added N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) to osmotically balanced ACSF (HEPES-ACSF), thereby increasing buffering capacity beyond that provided by bicarbonate/CO2. Water gain was markedly inhibited in HEPES-ACSF. After 3 h incubation in HEPES-ACSF at 35 degrees C, water gain was limited to that of fresh slices after 1 h recovery in ACSF at room temperature. The effect of HEPES in decreasing slice water gain was concentration dependent from 0.3 to 20 mM. The inhibition of water gain by HEPES suggests that tissue acidosis is a contributing factor in brain slice edema.

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Citations

Nov 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Craig J Dietrich, Martin Morad
Sep 5, 2006·Clinics in Perinatology·Karen I Fritz, Maria Delivoria-Papadopoulos
Mar 29, 2012·British Journal of Pharmacology·S HugelR Schlichter
Oct 7, 2014·Frontiers in Systems Neuroscience·Bryan M KrauseMatthew I Banks
Oct 24, 2014·Frontiers in Systems Neuroscience·Aeyal RazMatthew I Banks
Jan 25, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sabina HrabetováCharles Nicholson
Dec 15, 2017·Cephalalgia : an International Journal of Headache·Fan BuMinyan Wang
Nov 5, 2004·American Journal of Physiology. Cell Physiology·Robert W PutnamNicola A Ritucci
Aug 6, 2017·Brain Structure & Function·G SmolyakovD N Arvanitis
Jun 21, 2019·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·M Sanz CortesM A Belfort
Dec 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·David C Van Essen
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