Heptapeptide ligands against receptor-binding sites of influenza hemagglutinin toward anti-influenza therapy

Bioorganic & Medicinal Chemistry
Teruhiko MatsubaraToshinori Sato

Abstract

The initial attachment of influenza virus to cells is the binding of hemagglutinin (HA) to the sialyloligosaccharide receptor; therefore, the small molecules that inhibit the sugar-protein interaction are promising as HA inhibitors to prevent the infection. We herein demonstrate that sialic acid-mimic heptapeptides are identified through a selection from a primary library against influenza virus HA. In order to obtain lead peptides, an affinity selection from a phage-displayed random heptapeptide library was performed with the HAs of the H1 and H3 strains, and two kinds of the HA-binding peptides were identified. The binding of the peptides to HAs was inhibited in the presence of sialic acid, and plaque assays indicated that the corresponding N-stearoyl peptide strongly inhibited infections by the A/Aichi/2/68 (H3N2) strain of the virus. Alanine scanning of the peptides indicated that arginine and proline were responsible for binding. The affinities of several mutant peptides with single-amino-acid substitutions against H3 HA were determined, and corresponding docking studies were performed. A Spearman analysis revealed a correlation between the affinity of the peptides and the docking study. These results provide a practicable...Continue Reading

References

Oct 23, 1997·Structure·R C Wade
Jun 9, 2004·Drug Discovery Today·Robert C LadnerMark de Souza
Dec 1, 2007·Nature Reviews. Drug Discovery·Mark von Itzstein

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Citations

Jun 20, 2018·Chemical Communications : Chem Comm·Shuhei KosonoKazunobu Toshima
Dec 13, 2018·Future Medicinal Chemistry·Paola ChiodelliMarco Rusnati

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