HER 2 immunohistochemistry for breast cancer cell blocks can be used in the same way as that used for histological specimens

Diagnostic Cytopathology
Rieko NishimuraShinichi Tanaka

Abstract

Human epidermal growth factor receptor 2 (HER2) testing of samples from recurrent or metastatic breast cancer is recommended by the 2013 update of the American Society of Clinical Oncology/College of American Pathologists guidelines. Although cytological analysis can be applied to several types of metastatic lesions, the practical method for HER2 testing of cytological specimens is yet to be resolved. We conducted immunohistochemical (IHC) staining for HER2 in breast cancer cell blocks (CBs) and compared the results with those from the corresponding histological specimens. In cases of discrepancy between the two types of specimen, the bright-field HER2 dual in situ hybridization (DISH) assay was performed. CBs were prepared from 54 surgically excised breast cancers. The cells were fixed in 10% buffered formalin and embedded in paraffin. A Ventana BenchMark ULTRA (Roche Diagnostics) with anti-HER-2/neu (4B5) rabbit monoclonal primary antibody and INFORM HER2/neu Dual ISH DNA Probe Cocktail was used for the assays. Successful results were obtained in 52 of 54 CBs. Forty cases showed agreement between CBs and the histological specimens. No discrepancy was observed between the two types of specimens in cases where HER2 expression w...Continue Reading

References

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Citations

Aug 29, 2018·Cytopathology : Official Journal of the British Society for Clinical Cytology·Aruna Nambirajan, Deepali Jain
Apr 21, 2019·Clinical and Experimental Medicine·Liejun JiangHuayi Huang

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fluorescence microscopy
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biopsies
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