HER-2/neu gene amplification in stages I-IV breast cancer detected by fluorescent in situ hybridization

Genetics in Medicine : Official Journal of the American College of Medical Genetics
H F MarkP H Hsu

Abstract

Approximately 25-30% of breast and ovarian carcinomas have amplification of the HER-2/neu oncogene. The aim of the present study was to focus on HER-2/neu gene amplification in different clinical stages of breast cancer in order to (1) determine if fluorescent in situ hybridization (FISH) can be used to detect HER-2/neu gene amplification in different clinical stages of breast cancer, (2) establish whether HER-2/neu gene amplification characterizes a subset of breast cancer in each of these stages, and (3) determine whether a trend for correlation of amplification with the clinical stage of the disease can be detected using the FISH technology. A total of 40 specimens of formalin-fixed, paraffin-embedded breast cancer tissues were analyzed cytogenetically, in a blinded fashion, for HER-2/neu gene amplification using FISH and the Vysis LSI HER-2/neu Orange and CEP 17 Green DNA dual color probe. The criterion for "high amplification" was an amplification ratio of >4.0, that for "moderate amplification" a ratio between 2.1 and 4.0, and that for "low amplification" a ratio of 1.5-2.0. Using a cutoff point of > or =1.5, the overall frequency of HER-2/neu gene amplification among stage I tumors was 30% (3 out of 10). Of these, one-th...Continue Reading

Citations

Nov 10, 2013·Free Radical Biology & Medicine·Hye-Kyung Na, Young-Joon Surh
Dec 22, 1999·Experimental and Molecular Pathology·H F Mark
Jan 21, 2000·Experimental and Molecular Pathology·H F MarkD Feldman
Jul 20, 2002·Experimental and Molecular Pathology·Saori KanekoS Robert Young
Jul 20, 1999·Experimental and Molecular Pathology·H F MarkM Samy
Jul 20, 1999·Experimental and Molecular Pathology·A M AfifyH F Mark

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