HER2 FISH results in breast cancers with increased CEN17 signals using alternative chromosome 17 probes - reclassifying cases in the equivocal category

Histopathology
Maria-Anna HolzschuhGero Brockhoff

Abstract

HER2 testing of invasive breast cancer by in-situ hybridization guides therapy decisions. Probing HER2 and centromere of chromosome 17 (cen17) simultaneously is supposed to reveal both a potential HER2 gene amplification and polysomy 17. However, a considerable number of breast cancer patients with quasi polysomy 17 are considered 'equivocal', which is diagnostically meaningless. Moreover, patients with equivocal/false polysomic tumours are prevented from a potentially beneficial anti-HER2 treatment. Here we evaluated the RAI1, D17S122 and TP53 hybridization markers to indicate true polysomy reliably and to reclassify equivocal samples accurately as HER2-positive. Samples with (n = 82) and without (n = 52) increased cen17 copy numbers and 78 evidently HER2-amplified specimens were analysed using dual and triple marker hybridization probes. Selected putative polysomic samples were subjected to array-based comparative genomic hybridization (aCGH). We found that 37.8% samples with putative polysomy 17 did not show any gain in RAI1, D17S122 or TP53. Accordingly, aCGH revealed evidence for the presence of HER2/cen17 co-amplification rather than for true polysomy in those cases. Reflex testing using alternate 17p markers reclassified...Continue Reading

References

Mar 12, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K ParkS Han
Jul 23, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jorma IsolaJohn M S Bartlett
Dec 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Antonio C WolffUNKNOWN College of American Pathologists
Jan 10, 2008·Breast Cancer Research : BCR·Andrea SassenGero Brockhoff
Mar 12, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Edurne ArriolaJorge S Reis-Filho
May 19, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·I-Tien YehShelly R Gunn
Nov 26, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Shaheenah DawoodSharon H Giordano
Feb 19, 2010·Nature·Rameen BeroukhimMatthew Meyerson
Aug 11, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Edith A PerezRobert B Jenkins
Jun 24, 2011·Breast Cancer Research and Treatment·Zsuzsanna VargaChristian Öhlschlegel
Sep 29, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Chun Hing TseAllen M Gown
Jun 29, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Wedad M HannaGiuseppe Viale
Oct 2, 2013·Cancer Treatment Reviews·Edith A PerezJohn M S Bartlett
Oct 9, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Antonio C WolffUNKNOWN College of American Pathologists
Jan 15, 2014·Journal of Clinical Pathology·Cathy B Moelans, Paul J van Diest
Mar 31, 2015·Pathology, Research and Practice·Xiaohong PuQing Ye
Jul 18, 2015·American Journal of Clinical Pathology·Paul E Swanson, Hua Yang
Jul 18, 2015·American Journal of Clinical Pathology·Thomas H LongMegan L Troxell
Aug 1, 2015·Breast Cancer Research and Treatment·Min Hye JangSo Yeon Park
Feb 6, 2016·Genes, Chromosomes & Cancer·Vladimira KoudelakovaMarian Hajduch
Feb 20, 2016·Breast Cancer Research and Treatment·Yao-Shan FanIsildinha M Reis
Mar 25, 2016·Histopathology·Gero BrockhoffSven Hauke

❮ Previous
Next ❯

Citations

Apr 9, 2019·American Journal of Clinical Pathology·Adlin M Gordian-ArroyoGary H Tozbikian
Mar 7, 2020·International Journal of Surgical Pathology·Mieke R Van BockstalChristine Galant
Mar 30, 2018·Archives of Pathology & Laboratory Medicine·Brannan B GriffinLuis Z Blanco
Dec 21, 2021·International Journal of Cancer. Journal International Du Cancer·Anja Kathrin WegeGero Brockhoff

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.