HER2 Gene Amplification by Fluorescence In Situ Hybridization (FISH) Compared With Immunohistochemistry (IHC) in 122 Equivocal Gastric Cancer Cases

Applied Immunohistochemistry & Molecular Morphology : AIMM
Xiaoyan LiuWei Ding

Abstract

To determine the frequency of HER2/neu amplification and the relationship between HER2/neu expression and clinicopathologic features in gastric cancers scored immunohistochemistry (IHC) 2+. A total of 122 gastric cancer cases scored IHC 2+ were retrospectively analyzed for HER2 amplification by fluorescence in situ hybridization (FISH). The correlation between HER2/neu expression and clinicopathologic features was analyzed. HER2/neu gene was amplified in 17 out of the 122 gastric cancer samples. The concordance rate between IHC and FISH was 13.9%. HER2/neu status was correlated with the age of patients (P<0.05). Polysomy of CEP17 was demonstrated in 46 cases, 11 of which (23.91%) were amplified for HER2/neu. Within the subgroups, a correlation between CEP17 polysomy and the depth of invasion was observed (P<0.05). The results highlight the necessity of FISH test for further categorization when gastric cancer cases are scored 2+ by IHC. The value of HER2/neu for a potential role as a negative prognostic factor in the equivocal gastric cancer cases is limited.

References

May 10, 2006·BMC Cancer·B SchuellF Wrba
May 10, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Farin KamangarWilliam F Anderson
Feb 11, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Guido SauterMichael F Press
Feb 27, 2010·Oncology·Jan Trøst Jørgensen
Jul 29, 2010·Virchows Archiv : an International Journal of Pathology·Josef RüschoffH H Kreipe
Apr 8, 2011·World Journal of Gastroenterology : WJG·Shi-Yan YanYi-Qun Du
Apr 21, 2011·Current Opinion in Oncology·Sylvie Lorenzen, Florian Lordick
Apr 7, 2012·Journal of Cancer·Jan Trøst Jørgensen, Maria Hersom
Jun 22, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·W Q ShengX Du
Oct 15, 2013·Journal of Surgical Oncology·Sarah B FisherShishir K Maithel

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Citations

May 5, 2021·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Yingying XuLin Shen

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