Herboxidiene triggers splicing repression and abiotic stress responses in plants

BMC Genomics
Sahar AlshareefMagdy M Mahfouz


Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.


Sep 12, 2002·Annual Review of Plant Biology·Jian-Kang Zhu
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Gul Shad AliA S N Reddy
Feb 27, 2007·The Plant Journal : for Cell and Molecular Biology·Saiprasad G PalusaA S N Reddy
Jul 24, 2007·Nature Chemical Biology·Daisuke KaidaMinoru Yoshida
Jul 24, 2007·Nature Chemical Biology·Yoshihiko KotakeYoshiharu Mizui
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sascha LaubingerDetlef Weigel
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
May 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hiroaki Fujii, Jian-Kang Zhu
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Taishi UmezawaKazuo Shinozaki
Oct 28, 2009·Genome Research·Sergei FilichkinTodd C Mockler
Dec 29, 2009·Trends in Plant Science·Réka Tóth, Renier A L van der Hoorn
Apr 23, 2010·The Plant Journal : for Cell and Molecular Biology·Takashi Hirayama, Kazuo Shinozaki
May 22, 2010·The Plant Journal : for Cell and Molecular Biology·Xiaozhi RenZhizhong Gong
Oct 6, 2010·Genome Research·Angela N BrooksBrenton R Graveley
Dec 9, 2010·ACS Chemical Biology·Makoto HasegawaTamio Mizukami
Jan 25, 2011·Plant Signaling & Behavior·Paula Duque
Aug 16, 2011·Biochimica Et Biophysica Acta·Kentaro NakaminamiMotoaki Seki
Nov 3, 2011·Plant Physiology·A S N ReddyAndrea Barta
Jun 30, 2012·Trends in Plant Science·Naeem H SyedJohn W S Brown
Nov 6, 2012·Nature Reviews. Drug Discovery·Sophie BonnalJuan Valcárcel
Nov 23, 2012·Chembiochem : a European Journal of Chemical Biology·Yang GaoKazunori Koide
Apr 10, 2013·Blood·Youzhong Wan, Catherine J Wu
Apr 16, 2013·The Plant Journal : for Cell and Molecular Biology·Yanjie LuoXia Li
Jun 19, 2013·Proceedings of the National Academy of Sciences of the United States of America·Pengcheng WangJian-Kang Zhu
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Masanori OkamotoSean R Cutler
Nov 2, 2013·The Plant Cell·A S N ReddyAndrea Barta
Dec 5, 2013·The Journal of Biological Chemistry·Kerstin A EffenbergerMelissa S Jurica
Aug 27, 2014·Wiley Interdisciplinary Reviews. RNA·Jonathan M Howard, Jeremy R Sanford
Oct 1, 2014·International Journal of Molecular Sciences·Tiago M D CruzPaula Duque
Dec 3, 2014·Cold Spring Harbor Perspectives in Biology·Craig S Pikaard, Ortrun Mittelsten Scheid
Mar 19, 2015·Annual Review of Biochemistry·Yeon Lee, Donald C Rio
Apr 4, 2015·Current Opinion in Plant Biology·Sergei FilichkinTodd C Mockler
Jul 21, 2015·Current Opinion in Plant Biology·Giovanna CapovillaMarkus Schmid
Aug 20, 2015·The Plant Cell·John W S BrownMaria Kalyna
Oct 1, 2015·Nature Communications·Zhijuan WangXia Li
Dec 20, 2015·Trends in Biochemical Sciences·Panagiotis Papasaikas, Juan Valcárcel
Jul 22, 2016·Wiley Interdisciplinary Reviews. RNA·Kerstin A EffenbergerMelissa S Jurica
Jul 29, 2016·Wiley Interdisciplinary Reviews. RNA·Ruth Sperling
Sep 25, 2016·The Plant Journal : for Cell and Molecular Biology·Yu LingMagdy M Mahfouz


Feb 24, 2018·Journal of Experimental Botany·Yu LingMagdy M Mahfouz
May 1, 2019·Genome Biology·Haroon ButtMagdy M Mahfouz
Aug 2, 2019·Plant & Cell Physiology·Miku IshizawaKumi Sato-Nara
Jan 23, 2018·Frontiers in Plant Science·Misato Ohtani

Related Concepts

TAN 1609
Pladienolide B
Abscisic Acid, (E, Z)-(+, -)-Isomer
Serine-Arginine Splicing Factors
Epoxy Compounds
Fatty Alcohols
Early Promoters, Genetic
Primary RNA Transcript
RNA, Messenger, Splicing

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.