Hericium erinaceus isolectins recognize mucin-type O-glycans as tumor-associated carbohydrate antigens on the surface of K562 human leukemia cells

International Journal of Biological Macromolecules
Seonghun Kim

Abstract

In Hericium erinaceus mushroom fruiting body, two different lectin groups, HEL1 and HEL2, were identified by using peptide mass fingerprinting based on customized protein sequence databases derived from RNA-Seq data. The HEL2 group included four isoforms designated HEL2a-d. Codon-optimized genes encoding HEL1, HEL2a, and HEL2b were expressed in Escherichia coli to produce fully active soluble proteins designated rHEL1, rHEL2a, and rHEL2b. Interestingly, these lectins showed different molecular weights: approximately 15 kDa for rHEL1 and approximately 120 kDa for rHEL2a and rHEL2b under non-denaturing conditions. rHEL2a and rHEL2b exhibited agglutination activities, but rHEL1 did not show any agglutination activity toward animal erythrocytes. The hemagglutination activity of rHEL2 lectins was strongly inhibited by glycoproteins containing mucin-type O-glycans. Glycan array analysis and isothermal titration calorimetry revealed that rHEL2 isolectins interacted strongly with O-glycans harboring the core 1 O-glycan motif, Galβ(1,3)GalNAc. Moreover, the glycan binding specificities of rHEL2 isolectins were comparable to that of peanut agglutinin in their ability to recognize O-glycans attached to leukosialin as tumor-associated carb...Continue Reading

References

Sep 27, 2005·Critical Reviews in Food Science and Nutrition·Elvira González De Mejía, Valentin I Prisecaru
Sep 9, 2006·Cell·Kazuaki Ohtsubo, Jamey D Marth
Mar 30, 2007·Journal of Molecular Biology·Demetres D LeonidasNikos G Oikonomakos
Feb 25, 2011·Glycoconjugate Journal·Vishwanath B ChachadiBale M Swamy
Aug 13, 2013·Trends in Molecular Medicine·Salomé S PinhoCelso A Reis
Jan 21, 2014·Chemistry & Biology·Richard D Cummings, J Michael Pierce
Mar 13, 2014·Expert Review of Proteomics·Haitham A BadrChen-Zhong Li
Aug 22, 2014·Bioinformatics·Sanjay B AgravatDavid F Smith
Mar 3, 2015·Advances in Cancer Research·Matthew R KudelkaRichard D Cummings
Apr 10, 2015·International Journal of Molecular Sciences·Mohamed Ali Abol HassanJoe Tiralongo
Jun 16, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vassiliki I PeppaDemetres D Leonidas
Aug 21, 2015·Nature Reviews. Cancer·Salomé S Pinho, Celso A Reis
Nov 2, 2015·Applied Microbiology and Biotechnology·Jerica SabotičMarkus Künzler
Nov 5, 2015·Advances in Experimental Medicine and Biology·Patrizia Bottoni, Roberto Scatena
Oct 30, 2016·Current Protein & Peptide Science·Ram Sarup SinghJagat Rakesh Kanwar
Jun 10, 2017·International Journal of Molecular Sciences·Guillaume PoirouxPierre Rougé
Oct 11, 2017·International Journal of Biological Macromolecules·Seonghun Kim
Jan 27, 2018·Cell·Hiren J JoshiAdnan Halim

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