Herpes simplex virus 1 induces de novo phospholipid synthesis

Virology
Esther SutterPeter Wild

Abstract

Herpes simplex virus type 1 capsids bud at nuclear membranes and Golgi membranes acquiring an envelope composed of phospholipids. Hence, we measured incorporation of phospholipid precursors into these membranes, and quantified changes in size of cellular compartments by morphometric analysis. Incorporation of [³H]-choline into both nuclear and cytoplasmic membranes was significantly enhanced upon infection. [³H]-choline was also part of isolated virions even grown in the presence of brefeldin A. Nuclei expanded early in infection. The Golgi complex and vacuoles increased substantially whereas the endoplasmic reticulum enlarged only temporarily. The data suggest that HSV-1 stimulates phospholipid synthesis, and that de novo synthesized phospholipids are inserted into nuclear and cytoplasmic membranes to i) maintain membrane integrity in the course of nuclear and cellular expansion, ii) to supply membrane constituents for envelopment of capsids by budding at nuclear membranes and Golgi membranes, and iii) to provide membranes for formation of transport vacuoles.

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Citations

Jul 14, 2012·Virology·Peter WildKurt Tobler
Nov 5, 2016·Journal of Medical Virology·Yi ZhangMengzhou Zhou
Nov 10, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Wenbin YangDavid J Klumpp
Jan 11, 2017·Particle and Fibre Toxicology·Christine SattlerTobias Stoeger
Sep 23, 2018·Protein & Cell·Ying LvHongyu Deng
Nov 21, 2018·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Hui LiuWei Lu
Aug 28, 2018·F1000Research·Peter WildMathias Ackermann

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