Herpes Simplex Virus 1 MicroRNA miR-H8 Is Dispensable for Latency and Reactivation In Vivo.

Journal of Virology
Enrico R BarrozoD C Bloom

Abstract

The regulatory functions of 10 individual viral microRNAs (miRNAs) that are abundantly expressed from the herpes simplex virus 1 (HSV-1) latency-associated transcript (LAT) region remain largely unknown. Here, we focus on HSV-1 miRNA miR-H8, which is within the LAT 3p exon, antisense to the first intron of ICP0, and has previously been shown to target a host glycosylphosphatidylinositol (GPI)-anchoring pathway. However, the functions of this miRNA have not been assessed in the context of the viral genome during infection. Therefore, we constructed a recombinant virus lacking miR-H8 (17dmiR-H8) and compared it to the parental wild-type and rescue viruses to characterize phenotypic differences. In rabbit skin cells, 17dmiR-H8 exhibited only subtle reductions in viral yields. In contrast, we found significant decreases in both viral yields (8-fold) and DNA replication (9.9-fold) in murine neuroblastoma cells, while 17dmiR-H8 exhibited a 3.6-fold increase in DNA replication in differentiated human neuronal cells (Lund human mesencephalic [LUHMES] cells). These cell culture phenotypes suggested potential host- and/or neuron-specific roles for miR-H8 in acute viral replication. To assess whether miR-H8 plays a role in HSV latency or ...Continue Reading

References

Sep 1, 1987·The Journal of General Virology·F Sedarati, J G Stevens
Aug 27, 1971·Science·J G Stevens, M L Cook
Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shuang TangPhilip R Krause
Feb 26, 2010·Journal of Virology·Igor JurakDonald M Coen
Jan 1, 1998·Methods in Molecular Medicine·D C Bloom
Mar 30, 2012·Genome Research·Jian Lu, Andrew G Clark
Dec 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Te DuBernard Roizman
Aug 8, 2015·Current Pathobiology Reports·Emily R Feldman, Scott A Tibbetts
Feb 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Zhiyuan HanGuoying Zhou
Oct 21, 2016·Cell Reports·Jonatan EnkOfer Mandelboim
Apr 2, 2017·Journal of Neuroimmunology·Dane PhelanDavid C Bloom
Nov 28, 2018·Non-coding RNA·Maja Cokarić BrdovčakIgor Jurak
Dec 5, 2019·Proceedings of the National Academy of Sciences of the United States of America·Brett A HoffmanScott A Tibbetts
Apr 17, 2020·Journal of Virology·Enrico R BarrozoDavid C Bloom

❮ Previous
Next ❯

Citations

Jan 30, 2021·Frontiers in Microbiology·Juliette SavoretNathalie Chazal
May 21, 2021·Frontiers in Immunology·Luisa F DuartePablo A González

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.