Herpes Simplex Virus-1 qPCR in the Diagnosis of Lower Respiratory Tract Infections in Organ Transplant Recipients and Critically Ill Patients

American Journal of Clinical Pathology
Michelle N StramNahed Ismail

Abstract

To determine a quantitative herpes simplex virus (HSV) DNA threshold in lower respiratory tract specimens that correlates with positive viral culture and clinical outcomes. Bronchoalveolar lavage and bronchial wash samples from 53 HSV culture-positive and 61 culture-negative matched controls were tested using HSV-1 and HSV-2 quantitative polymerase chain reaction (qPCR). Median viral culture turnaround time was 21.8 days and 9.9 days for culture-negative and culture-positive specimens, respectively. Using an HSV-1 viral load threshold of 1.62 × 103 copies/mL, there was 93% agreement with viral culture. An HSV-1 viral load ≥1.3 × 104 copies/mL was associated with worse clinical outcome compared to a viral load <1.3 × 104 copies/mL (hazard ratio [HR] = 4.27, P = .017), and there was a trend of worse outcome compared to patients with undetectable HSV-1 DNA (HR = 1.60, P = .056). qPCR has clinical utility for rapid accurate identification of HSV-1 in lower respiratory tract specimens.

References

Aug 1, 1992·The American Review of Respiratory Disease·R K MerchantG W Hunninghake
Nov 1, 1996·The European Respiratory Journal·R J ByersE B Faragher
Aug 31, 2002·Journal of Clinical Microbiology·Karen C Carroll
Aug 24, 2006·JAMA : the Journal of the American Medical Association·Fujie XuLauri E Markowitz
Sep 28, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A M Simoons-SmitC M Vandenbroucke-Grauls
Jan 16, 2007·Clinical Microbiology Reviews·Diane S Leland, Christine C Ginocchio
Nov 3, 2007·Seminars in Respiratory and Critical Care Medicine·Robert P Baughman
Dec 18, 2008·Source Code for Biology and Medicine·Zoran BursacDavid W Hosmer
Jun 24, 2011·Radiology·Tomás Franquet
May 3, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C CostaR Cavallo
May 25, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Emilie FrobertFlorence Morfin
May 8, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Stephanie TraenPhilippe G Jorens
Mar 10, 2015·The Journal of Infection·Q LepillerF Stoll-Keller

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