Herpes simplex virus 1 VP22 regulates translocation of multiple viral and cellular proteins and promotes neurovirulence.

Journal of Virology
Michiko TanakaYasushi Kawaguchi

Abstract

Herpes simplex virus 1 (HSV-1) protein VP22, encoded by the UL49 gene, is a major virion tegument protein. In the present study, we showed that VP22 was required for efficient redistribution of viral proteins VP16, VP26, ICP0, ICP4, and ICP27 and of cellular protein Hsc-70 to the cytoplasm of infected cells. We found that two dileucine motifs in VP22, at amino acids 235 and 236 and amino acids 251 and 252, were necessary for VP22 regulation of the proper cytoplasmic localization of these viral and cellular proteins. The dileucine motifs were also required for proper cytoplasmic localization of VP22 itself and for optimal expression of viral proteins VP16, VP22, ICP0, UL41, and glycoprotein B. Interestingly, a recombinant mutant virus with alanines substituted for the dileucines at amino acids 251 and 252 had a 50% lethal dose (LD(50)) for neurovirulence in mice following intracerebral inoculation about 10(3)-fold lower than the LD(50) of the repaired virus. Furthermore, the replication and spread of this mutant virus in the brains of mice following intracerebral inoculation were significantly impaired relative to those of the repaired virus. The ability of VP22 to regulate the localization and expression of various viral and ce...Continue Reading

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Citations

Nov 28, 2012·Journal of Cancer Research and Clinical Oncology·Guishan JinFusheng Liu
Oct 27, 2015·Journal of Molecular Modeling·Gema L Ramírez-SalinasJ Correa-Basurto
Nov 10, 2013·Microbiology and Immunology·Naoto KoyanagiYasushi Kawaguchi
Jun 22, 2016·Viral Immunology·Jianyun LiuRandy R Brutkiewicz
Mar 10, 2017·Journal of Virology·Nabil El BilaliRoger Lippé
Aug 28, 2020·Frontiers in Microbiology·Liping WuXiaoyue Chen
May 12, 2016·Experimental and Therapeutic Medicine·Xian YuYan Wang
May 26, 2018·Journal of Virology·Marielle LebrunCatherine Sadzot-Delvaux
Feb 26, 2019·Medical Microbiology and Immunology·Markus StempelMelanie M Brinkmann
Sep 12, 2017·The Journal of Clinical Investigation·Naoto KoyanagiYasushi Kawaguchi
Dec 31, 2020·Viruses·Christos DogrammatzisMaria Kalamvoki
Nov 23, 2021·PLoS Pathogens·Shirley E BraspenningWerner J D Ouwendijk

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