PMID: 9525627Apr 3, 1998Paper

Herpes simplex virus DNA cleavage and packaging: association of multiple forms of U(L)15-encoded proteins with B capsids requires at least the U(L)6, U(L)17, and U(L)28 genes

Journal of Virology
B Salmon, Joel D Baines

Abstract

The U(L)15 gene of herpes simplex virus (HSV) is one of several genes required for the packaging of viral DNA into intranuclear B capsids to produce C capsids that become enveloped at the inner nuclear membrane. A rabbit antiserum directed against U(L)15-encoded protein recognized three proteins with apparent Mrs of 79,000, 80,000, and 83,000 in highly purified B capsids. The 83,000-Mr protein was detected in type C capsids and comigrated with the product of a U(L)15 cDNA transcribed and translated in vitro. The 83,000- and 80,000-Mr proteins were readily detected in purified virions. Inasmuch as (i) none of these proteins were detectable in capsids purified from cells infected with HSV-1(deltaU(L)15), a virus lacking an intact U(L)15 gene, and (ii) corresponding proteins in capsids purified from cells infected with a recombinant virus [HSV-1(R7244), containing a 20-codon tag at the 3' end of U(L)15] were decreased in electrophoretic mobility relative to the wild-type proteins, we conclude that the proteins with apparent Mrs of 83,000, 80,000, and 79,000 are products of U(L)15 with identical C termini. The 79,000-, 80,000-, and 83,000-Mr proteins remained associated with B capsids in the presence of 0.5 M guanidine HCl and rema...Continue Reading

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Citations

May 16, 2007·Molecular Microbiology·Peixuan Guo, Tae Jin Lee
Jan 1, 2010·Journal of Virology·Amy L HarperJohn W Wills
Feb 11, 1999·Journal of Virology·A Dasgupta, D W Wilson
Nov 6, 2018·Frontiers in Microbiology·Francisco J IbáñezPablo A González
Jan 20, 2000·Virology·A E ReynoldsJ D Baines
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·A JacobsC Fraefel

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