PMID: 9525593Apr 3, 1998Paper

Herpes simplex virus DNA packaging without measurable DNA synthesis

Journal of Virology
G A ChurchD W Wilson

Abstract

Herpes simplex virus (HSV) type 1 DNA synthesis and packaging occur within the nuclei of infected cells; however, the extent to which the two processes are coupled remains unclear. Correct packaging is thought to be dependent upon DNA debranching or other repair processes, and such events commonly involve new DNA synthesis. Furthermore, the HSV UL15 gene product, essential for packaging, nevertheless localizes to sites of active DNA replication and may link the two events. It has previously been difficult to determine whether packaging requires concomitant DNA synthesis due to the complexity of these processes and of the viral life cycle; however, we have recently described a model system which simplifies the study of HSV assembly. Cells infected with HSV strain tsProt.A accumulate unpackaged capsids at the nonpermissive temperature of 39 degrees C. Following release of the temperature block, these capsids proceed to package viral DNA in a single, synchronous wave. Here we report that, when DNA replication was inhibited prior to release of the temperature block, DNA packaging and later events in viral assembly nevertheless occurred at near-normal levels. We conclude that, under our conditions, HSV DNA packaging does not require...Continue Reading

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Citations

Aug 30, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Sandra LoretRoger Lippé
Nov 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Thejaswi NagarajuBill Sugden
Sep 16, 2006·Journal of Virology·Gaudeline Rémillard-LabrosseRoger Lippé
Jun 20, 2001·The Journal of General Virology·A Dasgupta, D W Wilson
Feb 11, 1999·Journal of Virology·A Dasgupta, D W Wilson

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