Herpes simplex virus gE/gI must accumulate in the trans-Golgi network at early times and then redistribute to cell junctions to promote cell-cell spread

Journal of Virology
Aaron Farnsworth, David C Johnson

Abstract

Herpes simplex virus (HSV) glycoprotein heterodimer gE/gI is necessary for virus spread in epithelial and neuronal tissues. Deletion of the relatively large gE cytoplasmic (CT) domain abrogates the ability of gE/gI to mediate HSV spread. The gE CT domain is required for the sorting of gE/gI to the trans-Golgi network (TGN) in early stages of virus infection, and there are several recognizable TGN sorting motifs grouped near the center of this domain. Late in HSV infection, gE/gI, other viral glycoproteins, and enveloped virions redistribute from the TGN to epithelial cell junctions, and the gE CT domain is also required for this process. Without the gE CT domain, newly enveloped virions are directed to apical surfaces instead of to cell junctions. We hypothesized that the gE CT domain promotes virus envelopment into TGN subdomains from which nascent enveloped virions are sorted to cell junctions, a process that enhances cell-to-cell spread. To characterize elements of the gE CT domain involved in intracellular trafficking and cell-to-cell spread, we constructed a panel of truncation mutants. Specifically, these mutants were used to address whether sorting to the TGN and redistribution to cell junctions are necessary, and suffic...Continue Reading

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Citations

Oct 17, 2008·Nature Reviews. Microbiology·Quentin Sattentau
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Barbara BerarducciAnn M Arvin
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jun HanJohn W Wills
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Apr 3, 2009·Current Opinion in HIV and AIDS·Nicola Martin, Quentin Sattentau

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