Herpes simplex virus ICP27 protein directly interacts with the nuclear pore complex through Nup62, inhibiting host nucleocytoplasmic transport pathways.

The Journal of Biological Chemistry
Poonam MalikEric C Schirmer

Abstract

The herpes simplex virus ICP27 protein is important for the expression and nuclear export of viral mRNAs. Although several binding sites have been mapped along the ICP27 sequence for various RNA and protein partners, including the transport receptor TAP of the host cell nuclear transport machinery, several aspects of ICP27 trafficking through the nuclear pore complex remain unclear. We investigated if ICP27 could interact directly with the nuclear pore complex itself, finding that ICP27 directly binds the core nucleoporin Nup62. This is confirmed through co-immunoprecipitation and in vitro binding assays with purified components. Mapping with ICP27 deletion and point mutants further shows that the interaction requires sequences in both the N and C termini of ICP27. Expression of wild type ICP27 protein inhibited both classical, importin α/β-dependent and transportin-dependent nuclear import. In contrast, an ICP27 point mutant that does not interact with Nup62 had no such inhibitory effect. We suggest that ICP27 association with Nup62 provides additional binding sites at the nuclear pore for ICP27 shuttling, thus supporting ICP27-mediated transport. We propose that ICP27 competes with some host cell transport receptors for bindi...Continue Reading

References

Feb 20, 1992·Nature·S Piñol-Roma, G Dreyfuss
Dec 1, 1982·The Journal of Cell Biology·L GeraceC Kondor-Koch
May 1, 1995·The Journal of Cell Biology·H Siomi, G Dreyfuss
Sep 1, 1994·Antimicrobial Agents and Chemotherapy·L S TzouvelekisA F Mentis
Jan 6, 1995·The Journal of Biological Chemistry·C MacaulayD J Forbes
Nov 1, 1994·AIDS Research and Human Retroviruses·A L Frazier, J V Garcia
Jul 1, 1994·Genes & Development·B E Meyer, M H Malim
Dec 1, 1994·Molecular and Cellular Biology·J FlachP A Silver
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A PhelanJ B Clements
Sep 20, 1996·Cell·V W PollardG Dreyfuss
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·N BonifaciG Blobel
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y LiR M Krug
Feb 23, 2000·Journal of Virology·T M Soliman, S J Silverstein
May 29, 2000·Science·S R Wente
Jul 12, 2001·Proceedings of the National Academy of Sciences of the United States of America·J M PetersenJ E Dahlberg
Sep 12, 2001·The Journal of Biological Chemistry·I Schmitt, L Gerace
Sep 5, 2002·Journal of Virology·Henri GruffatAlain Sergeant

❮ Previous
Next ❯

Citations

Aug 21, 2013·Viruses·Valerie Le Sage, Andrew J Mouland
Feb 13, 2015·Viruses·Vladimir Majerciak, Zhi-Ming Zheng
Nov 29, 2014·The FEBS Journal·Bastien CautainWolfgang Link
Dec 3, 2013·Traffic·Melanie L YarbroughBeatriz M A Fontoura
Nov 27, 2015·Virology Journal·Chaoyue LiuXiaoyue Chen
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Gilad SivanBernard Moss
Nov 12, 2014·PloS One·Poonam MalikEric C Schirmer
Feb 10, 2017·Molecular & Cellular Proteomics : MCP·Katarzyna KulejMatthew D Weitzman
Jul 3, 2013·Molecular & Cellular Proteomics : MCP·Sandie MunierNadia Naffakh
Jan 22, 2019·Critical Reviews in Microbiology·Wen MengHwa-Chain Robert Wang
Sep 17, 2020·Viruses·Jón Pol GalesMaria Dimitrova
Jun 8, 2021·The Journal of Biological Chemistry·Qingtang ShenAlexander F Palazzo
Jul 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kalanghad Puthankalam SrinivasAngus C Wilson

❮ Previous
Next ❯

Methods Mentioned

BETA
FCS
co-immunoprecipitation
scraping
immunoprecipitations
Pull-down
transfection
fluorescence microscopy
glycosylation
immunoprecipitation
co-immunoprecipitations

Software Mentioned

AxioVision
Adobe Photoshop
Metamorph
IPLab4
AutoquantX

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.