PMID: 9188566Jul 1, 1997Paper

Herpes simplex virus type 1 glycoprotein K is not essential for infectious virus production in actively replicating cells but is required for efficient envelopment and translocation of infectious virions from the cytoplasm to the extracellular space

Journal of Virology
S JayachandraK G Kousoulas

Abstract

We characterized the glycoprotein K (gK)-null herpes simplex virus type 1 [HSV-1] (KOS) delta gK and compared it to the gK-null virus HSV-1 F-gKbeta (L. Hutchinson et al., J. Virol. 69:5401-5413, 1995). delta gK and F-gKbeta mutant viruses produced small plaques on Vero cell monolayers at 48 h postinfection. F-gKbeta caused extensive fusion of 143TK cells that was sensitive to melittin, a specific inhibitor of gK-induced cell fusion, while delta gK virus did not fuse 143TK cells. A recombinant plasmid containing the truncated gK gene specified by F-gKbeta failed to rescue the ICP27-null virus KOS (d27-1), while a plasmid with the delta gK deletion rescued the d27-1 virus efficiently. delta gK virus yield was approximately 100,000-fold lower in stationary cells than in actively replicating Vero cells. The plaquing efficiencies of delta gK and F-gKbeta virus stocks on VK302 cells were similar, while the plaquing efficiency of F-gKbeta virus stocks on Vero cells was reduced nearly 10,000-fold in comparison to that of delta gK virus. Mutant delta gK and F-gKbeta infectious virions accumulated within Vero and HEp-2 cells but failed to translocate to extracellular spaces. delta gK capsids accumulated in the nuclei of Vero but not HEp...Continue Reading

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Citations

Dec 10, 1999·Journal of Virology·R J RollerD DeSalvo
Sep 29, 2005·Journal of Virology·Helene LeuzingerPeter Wild
Jan 15, 2010·The Journal of General Virology·Gabriel André Leiva-TorresAngela Pearson
Apr 16, 2011·Nature Reviews. Microbiology·David C Johnson, Joel D Baines
Sep 27, 2012·Viruses·Walid Azab, Abuelyazeed El-Sheikh
Jan 31, 1998·The Journal of Biological Chemistry·C Mo, T C Holland
Aug 27, 2015·Virology Journal·Robyn N HallTimothy J Mahony
Sep 24, 2015·Viruses·Danielle J OwenStephen C Graham
Feb 10, 2012·Journal of Virology·Dmitry V ChouljenkoKonstantin G Kousoulas
May 9, 2000·Journal of Virology·P DietzT C Mettenleiter
Jul 11, 2000·Journal of Virology·B G KluppT C Mettenleiter
Aug 23, 2020·International Journal of Molecular Sciences·Imran Ahmad, Duncan W Wilson
Dec 31, 2020·Viruses·Christos DogrammatzisMaria Kalamvoki

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