PMID: 9524194May 9, 1998Paper

Herpes simplex virus type 1-infected human embryonic lung cells studied by optimized immunogold cryosection electron microscopy

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
H L Jensen, B Norrild

Abstract

Herpes simplex virus type 1 (HSV-1) is a common human pathogen of skin and mucous membranes and is potentially dangerous when the infection is disseminated. Viral morphogenesis, especially the mechanism of viral envelopment and the exact pathway for processing and transport of HSV-1 glycoproteins, is still unclear. We report the results of optimized immunogold-labeled cryosection electron microscopy of HSV-1-infected cultured human fibroblasts (MRC-5). The simplified method presented has proved necessary to obtain reproducible results on cellular distribution of viral glycoproteins. It is now possible to demonstrate the viral glycoprotein gD-1, but not gC-1, in the nuclear membranes and to demonstrate gD-1- and gC-1-labeled viral particles in the perinuclear space, and to show the fate of the viral particles in the endoplasmic reticulum and Golgi area in infected cells.

References

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Jul 1, 1995·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H L JensenB Norrild
May 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H JensenB Norrild

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Citations

Jan 30, 2002·Micron : the International Research and Review Journal for Microscopy·Peter WildMonika Engels
Apr 18, 2008·Journal of Virology·Barbara KluppThomas C Mettenleiter
Jan 12, 2007·Journal of Virology·Joel D BainesMichael Marko
Sep 29, 2005·Journal of Virology·Helene LeuzingerPeter Wild
Aug 5, 2009·Virus Research·Thomas C MettenleiterHarald Granzow
Nov 25, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Helle Lone Jensen, Bodil Norrild
Mar 15, 2002·Applied Immunohistochemistry & Molecular Morphology : AIMM·Helle L Jensen, Bodil Norrild
Nov 5, 2019·Journal of Histotechnology·Joshua LloydQin Su

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