Herpes simplex virus VP16, but not ICP0, is required to reduce histone occupancy and enhance histone acetylation on viral genomes in U2OS osteosarcoma cells.

Journal of Virology
Meaghan H HancockJames R Smiley

Abstract

The herpes simplex virus (HSV) genome rapidly becomes associated with histones after injection into the host cell nucleus. The viral proteins ICP0 and VP16 are required for efficient viral gene expression and have been implicated in reducing the levels of underacetylated histones on the viral genome, raising the possibility that high levels of underacetylated histones inhibit viral gene expression. The U2OS osteosarcoma cell line is permissive for replication of ICP0 and VP16 mutants and appears to lack an innate antiviral repression mechanism present in other cell types. We therefore used chromatin immunoprecipitation to determine whether U2OS cells are competent to load histones onto HSV DNA and, if so, whether ICP0 and/or VP16 are required to reduce histone occupancy and enhance acetylation in this cell type. High levels of underacetylated histone H3 accumulated at several locations on the viral genome in the absence of VP16 activation function; in contrast, an ICP0 mutant displayed markedly reduced histone levels and enhanced acetylation, similar to wild-type HSV. These results demonstrate that U2OS cells are competent to load underacetylated histones onto HSV DNA and uncover an unexpected role for VP16 in modulating chroma...Continue Reading

References

Apr 1, 1991·The Journal of General Virology·R A Harris, C M Preston
Dec 1, 1987·Journal of Virology·J G Spivack, N W Fraser
Dec 1, 1987·The Journal of General Virology·J RussellC M Preston
May 1, 1985·Molecular and Cellular Biology·M P Quinlan, D M Knipe
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·I H Gelman, S Silverstein
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·W Gibson, B Roizman
May 1, 1980·Journal of Virology·G H CohenR J Eisenberg
Nov 1, 1980·The Journal of General Virology·S S Leinbach, W C Summers
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R D KlemmR Tjian
Jul 28, 1995·The Journal of Biological Chemistry·M Garcia-RamirezJ Ausio
Jun 25, 1998·Molecular and Cellular Biology·N KobayashiA J Berk
Jan 21, 2000·The Journal of General Virology·C M Preston
Aug 11, 2001·Science·T Jenuwein, C D Allis
Feb 8, 2003·Current Biology : CB·Sevinci Memedula, Andrew S Belmont
Dec 6, 2003·The EMBO Journal·Gerhard MittlerMichael Meisterernst
Mar 12, 2004·The EMBO Journal·Margaret KastenBradley R Cairns
Jun 15, 2004·Journal of Virology·Patrick LomonteAlberto L Epstein

❮ Previous
Next ❯

Citations

Jul 1, 2011·Journal of Virology·Bich Ngoc TranChoy Leong Hew
Mar 25, 2011·Herpesviridae·Rhiannon R Penkert, Robert F Kalejta
Jul 19, 2013·Viruses·Zachary WatsonDavid Bloom
Jul 19, 2013·Viruses·Kristen L Conn, Luis M Schang
Dec 23, 2011·Biology of the Cell·Nina Van OpdenboschGerlinde R Van de Walle
Mar 29, 2014·Reviews in Medical Virology·Shanshan LiYi Zheng
Dec 15, 2012·The Journal of General Virology·Chris Boutell, Roger D Everett
Mar 21, 2018·The Journal of Biological Chemistry·Dai HatakeyamaTakashi Kuzuhara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.