PMID: 9445042Jan 28, 1998Paper

Herpesvirus entry mediator HVEM mediates cell-cell spread in BHK(TK-) cell clones

Journal of Virology
R J Roller, D Rauch

Abstract

95-19 and U(S)11c119.3 are BHK(TK-)-derived cell lines that are highly resistant to postattachment entry of herpes simplex virus type 1 (HSV-1) and HSV-2 but not to later steps in single-step replication. The resistance properties of these two cell types are not identical. U(S)11c119.3 cells are fully susceptible to pseudorabies virus (PRV), as shown by single-step growth experiments, whereas 95-19 cells are resistant to entry of free PRV but not to entry by cell-cell spread. We have tested the ability of HVEM to overcome the block to infection in both cell lines following transient and stable transfection. HVEM was able to mediate entry of free HSV-1 into both cell lines, as shown by an increase in the number of beta-galactosidase-expressing cells in cultures transiently transfected with an HVEM expression plasmid and infected with lacZ-expressing HSV-1. In stably transfected 95-19 cells, HVEM enhanced infection by free HSV-1, as shown by an increase in the number of infectious centers obtained following infection. In both cell types, HVEM strongly enhanced entry of HSV-1 and HSV-2 by cell-cell spread, suggesting that HVEM can function as an entry mediator both in entry of free virus and in entry by cell-cell spread.

References

Mar 1, 1992·The Journal of Cell Biology·M T ShiehP G Spear
Oct 1, 1991·Journal of Virology·I Rauh, T C Mettenleiter
Apr 1, 1991·The Journal of General Virology·C A MacLeanD J McGeoch
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A O Fuller, P G Spear
Nov 1, 1995·Journal of Virology·K S DingwellD C Johnson
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·R BrandimartiG Campadelli-Fiume

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Citations

Jan 25, 2003·Antimicrobial Agents and Chemotherapy·Sara SinhaBetsy C Herold
Oct 31, 2007·FEBS Letters·Johanna KeyserKarin Moelling
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·A JacobsC Fraefel
Oct 7, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hyunjung BaekHeechung Kwon

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