HES1 and HES5 are dispensable for cartilage and endochondral bone formation

Cells, Tissues, Organs
Camilla KarlssonAnders Lindahl

Abstract

Notch signalling, via its downstream mediators HES1 and HES5, regulates development of several different tissues. In vitro studies suggest that these genes are also involved in chondrogenesis and endochondral bone formation. In order to investigate the importance of HES1 and HES5 for these developmental processes, mice lacking chondrogenic expression of HES1 and HES5 were constructed by interbreeding HES5(-/-) mice homozygous for the floxed HES1 allele (HES1(flox/flox)) with COL2A1-Cre transgenic mice, creating conditional HES1;HES5 double mutant mice. The formation of cartilage and endochondral bone was studied in these mice using histological and immunohistochemical stainings, including Alcian Blue van Gieson, Safranin-O, modified Mallory Aniline Blue, tartrate-resistant acid phosphatase and collagen type II stainings. The mice were also studied using several different morphometrical analyses and the differentiation potential of the chondrocytes was evaluated in vitro. Unexpectedly, the conditional HES1;HES5 double mutant mice did not display impaired development of cartilage or endochondral bone. Lack of altered phenotype in the conditional HES1;HES5 double mutant mice can be explained either by the HES1 and HES5 genes not b...Continue Reading

Citations

Aug 27, 2011·Cell Death and Differentiation·R HallerJ Rohwedel
Nov 19, 2010·The Journal of Biological Chemistry·Stefano ZanottiErnesto Canalis
Jul 16, 2016·Seminars in Cell & Developmental Biology·William E SamsaGuang Zhou
Apr 14, 2016·Endocrine Reviews·Stefano Zanotti, Ernesto Canalis
Feb 26, 2020·Biomolecules·Jennifer T ZiebaYangjin Bae

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