Hesperetin post-treatment prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating PI3K/Akt signaling pathway

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Shangfei HeAihua Chen

Abstract

Hesperidin (HES), a citrus fruit extract, has beneficial effects on various ischemia/reperfusion (I/R) models. Here, we investigated the possible positive effect of hesperetin (HPT), an active metabolite of HES, and identified the potential molecular mechanisms involved in cardiomyocytes H/R-induced injury. To construct the cardiomyocyte model of hypoxia/reoxygenation (H/R) injury, cultured neonatal rat cardiomyocytes were subjected to 3h of hypoxia followed by 3h of reoxygenation. Cell viability and apoptosis were detected. The levels of Apoptosis-related proteins and PI3K/Akt proteins were detected by western blot. Our results showed that HPT post-treatment significantly inhibited apoptosis by elevating the expression of Bcl-2, decreasing the expression of Bax and cleaved caspase-3, and diminished the apoptotic cardiomyocytes ratio. Mechanism studies demonstrated that HPT post-treatment up-regulated the expression levels of p-PI3K, and p-Akt. Co-treatment of the cardiomyocytes with the PI3K/Akt-specific inhibitor LY294002 blocked the HPT-induced cardioprotective effects. Taken together, these data suggested that HPT post-treatment prevented cardiomyocytes from H/R injury in vitro most likely through the activation of PI3K/Akt...Continue Reading

Citations

Aug 16, 2019·Drug and Chemical Toxicology·Ramin RezaeeMahmoud Hashemzaei
Feb 14, 2020·Experimental Biology and Medicine·Vaishali AggarwalGautam Sethi
Jul 10, 2019·Current Pharmaceutical Design·Ying TanMaolong Dong
Dec 7, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Tongtong XuXiangwei Lv
Aug 28, 2020·Frontiers in Physiology·Yu-Min ZhangRu-Xing Wang
Jun 12, 2021·Cardiovascular Toxicology·Shafreena Shaukat AliWan Amir Nizam Wan Ahmad

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