Hesperidin Attenuates Ultraviolet B-Induced Apoptosis by Mitigating Oxidative Stress in Human Keratinocytes

Biomolecules & Therapeutics
Susara Ruwan Kumara Madduma HewageJin Won Hyun

Abstract

Human skin cells undergo pathophysiological processes via generation of reactive oxygen species (ROS) upon excessive exposure to ultraviolet B (UVB) radiation. This study investigated the ability of hesperidin (C28H34O15) to prevent apoptosis due to oxidative stress generated through UVB-induced ROS. Hesperidin significantly scavenged ROS generated by UVB radiation, attenuated the oxidation of cellular macromolecules, established mitochondrial membrane polarization, and prevented the release of cytochrome c into the cytosol. Hesperidin downregulated expression of caspase-9, caspase-3, and Bcl-2-associated X protein, and upregulated expression of B-cell lymphoma 2. Hesperidin absorbed wavelengths of light within the UVB range. In summary, hesperidin shielded human keratinocytes from UVB radiation-induced damage and apoptosis via its antioxidant and UVB absorption properties.

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Citations

Feb 8, 2018·Oxidative Medicine and Cellular Longevity·Lucian HritcuDamião Pergentino de Sousa
Mar 20, 2020·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Denisa SkarupovaAlena Rajnochova Svobodova
Aug 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dahee KimChoongho Lee
May 8, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Mao-Qiang ManPeter M Elias
Oct 21, 2018·BMC Pharmacology & Toxicology·Sabah AnsarSherifa S Hamed
Sep 12, 2018·Journal of Cosmetic Dermatology·Sylvie BoisnicViolaine Jabbour
Nov 3, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Li-Ying ZhangYong-Qi Liu

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Methods Mentioned

BETA
ESR
electrophoresis
enzyme-linked immunosorbent assay
ELISA
flow cytometry
DNA fragmentation assay
fluorescence microscopy

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