Hesperidin Inhibits Vascular Formation by Blocking the AKT/mTOR Signaling Pathways

Preventive Nutrition and Food Science
Gi Dae Kim

Abstract

Hesperidin has been shown to possess a potential inhibitory effect on vascular formation in endothelial cells. However, the fundamental mechanism for the anti-angiogenic activity of hesperidin is not fully understood. In the present study, we evaluated whether hesperidin has anti-angiogenic effects in mouse embryonic stem cell (mES)-derived endothelial-like cells, and human umbilical vascular endothelial cells (HUVECs), and evaluated their mechanism via the AKT/mammalian target of rapamycin (mTOR) signaling pathway. The endothelial cells were treated with several doses of hesperidin (12.5, 25, 50, and 100 μM) for 24 h. Cell viability and vascular formation were analyzed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and tube formation assay, respectively. Alteration of the AKT/mTOR signaling in vascular formation was analyzed by western blot. In addition, a mouse aortic ring assay was used to determine the effect of hesperidin on vascular formation. There were no differences between the viability of mES-derived endothelial-like cells and HUVECs after hesperidin treatment. However, hesperidin significantly inhibited cell migration and tube formation of HUVECs (P<0.05) and suppressed sprouting of microvess...Continue Reading

References

Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Feb 1, 2000·Biochemical and Biophysical Research Communications·E A KrugerW D Figg
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Francesca TosettiAdriana Albini
Jun 29, 2002·Circulation Research·Ichiro Shiojima, Kenneth Walsh
Jul 18, 2002·The Journal of Nutritional Biochemistry·Yihai CaoEbba Bråkenhielm
Jun 21, 2003·The Journal of Biological Chemistry·Xianhe BaiJack L Arbiser
Oct 2, 2003·The Journal of Nutrition·Gary R Beecher
Jan 9, 2004·Oncogene·Kafi N MeadowsKevin M Pumiglia
Oct 19, 2004·Cancer Letters·Thambi Dorai, Bharat B Aggarwal
Jun 2, 2005·Nature Reviews. Cancer·Gillian M TozerBruce C Baguley
Oct 29, 2005·Journal of Clinical Pathology·E CristiC Rabitti
May 20, 2006·Medicinal Research Reviews·P FrescoM P M Marques
Sep 28, 2007·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·H J ParkJ-H Chung
May 14, 2008·Current Medicinal Chemistry·M HwangB Lu
Jul 3, 2008·Journal of Agricultural and Food Chemistry·O Benavente-García, J Castillo
Nov 5, 2008·Cancer Biology & Therapy·Shao-Hua ChenWilliam M F Lee
Jul 28, 2009·Journal of Thrombosis and Haemostasis : JTH·X Li, L Claesson-Welsh
Oct 30, 2009·Current Opinion in Genetics & Development·Claudio Areias FrancoHolger Gerhardt
Oct 30, 2009·The Journal of Biological Chemistry·Kathryn G FosterDiane C Fingar
Nov 12, 2009·International Journal of Cancer. Journal International Du Cancer·Michael S GordonGerry Kato
Nov 19, 2009·Journal of Agricultural and Food Chemistry·Jaiprakash R PatilBhimanagouda S Patil
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J HeuerH Spielmann
Dec 24, 2011·Nature Protocols·Marianne BakerKairbaan Hodivala-Dilke
Aug 15, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Gi Dae KimSang Kook Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.