Hetero-fertilization together with failed egg-sperm cell fusion supports single fertilization involved in in vivo haploid induction in maize

Journal of Experimental Botany
Xiaolong TianShaojiang Chen

Abstract

In vivo doubled-haploid technology is widely applied in commercial maize breeding programs because of its time-saving and cost-reducing features. The production of maize haploids primarily depends on the use of Stock6-derived haploid inducer lines. Although the gene underlying haploid induction, MTL/ZmPLA1/NLD, was cloned recently, the mechanism of haploid induction is still unknown. Hetero-fertilization can occur via a single fertilization, which provides a means to investigate single-fertilization events by studying the hetero-fertilization phenomenon. In this study, we found that the hetero-fertilization rate increased significantly when female maize lines were first individually crossed with pollen from the inducer CAU5 in dual-pollination experiments 4 h before a second pollination with common lines. We also examined embryogenesis during haploid induction by confocal laser-scanning microscopy and observed single-fertilized ovules, indicating that single fertilization occurred during haploid induction. We therefore postulate that both single fertilization and chromosome elimination contribute to haploid induction in maize. We also propose a scheme for the formation of hetero-fertilized and haploid kernels. Our results provi...Continue Reading

References

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Citations

Jul 26, 2019·Plant Biotechnology Journal·Chenxu LiuShaojiang Chen
Sep 27, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Vijay ChaikamPrasanna M Boddupalli
Jun 10, 2020·Nature Plants·Nathanaël M A JacquierThomas Widiez
Dec 7, 2018·Journal of Integrative Plant Biology·Weibin SongJinsheng Lai
Dec 21, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kamila KalinowskaAndreas Houben
Mar 6, 2019·Nature Biotechnology·Timothy KelliherQiudeng Que
May 16, 2020·Plants·Henrique Uliana TrentinThomas Lübberstedt

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Methods Mentioned

BETA
nuclear magnetic resonance
NMR
genotyping

Software Mentioned

Pro Plus
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