Heterochromatin protein 1 (HP1) is intrinsically required for post-transcriptional regulation of Drosophila Germline Stem Cell (GSC) maintenance

Scientific Reports
Assunta Maria CasaleLucia Piacentini

Abstract

A very important open question in stem cells regulation is how the fine balance between GSCs self-renewal and differentiation is orchestrated at the molecular level. In the past several years much progress has been made in understanding the molecular mechanisms underlying intrinsic and extrinsic controls of GSC regulation but the complex gene regulatory networks that regulate stem cell behavior are only partially understood. HP1 is a dynamic epigenetic determinant mainly involved in heterochromatin formation, epigenetic gene silencing and telomere maintenance. Furthermore, recent studies have revealed the importance of HP1 in DNA repair, sister chromatid cohesion and, surprisingly, in positive regulation of gene expression. Here, we show that HP1 plays a crucial role in the control of GSC homeostasis in Drosophila. Our findings demonstrate that HP1 is required intrinsically to promote GSC self-renewal and progeny differentiation by directly stabilizing the transcripts of key genes involved in GSCs maintenance.

References

Jan 11, 1991·Nucleic Acids Research·S R HaynesA L Beyer
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J C EissenbergS C Elgin
Dec 1, 1990·Genes & Development·Dennis M McKearin, Allan Spradling
Jan 1, 1988·Methods in Enzymology·R BrimacombeP Maly
Oct 28, 1994·Science·Lynn Cooley, William E Theurkauf
Mar 12, 1993·Cell·Allan Spradling
Jan 1, 1997·Annual Review of Genetics·M de CuevasAllan Spradling
Dec 9, 1998·Molecular Cell·Laura FantiSergio Pimpinelli
Dec 22, 1998·Mechanisms of Development·P Rørth
Oct 29, 1999·Genes & Development·J Sonoda, R P Wharton
Nov 24, 1999·Nature Cell Biology·M Asaoka-TaguchiS Kobayashi
Nov 2, 2001·Nature·Allan SpradlingT Kai
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 23, 2002·Trends in Genetics : TIG·Marvin WickensRoy Parker
Dec 3, 2002·Nature Reviews. Genetics·Haifan Lin
May 21, 2003·The Journal of Cell Biology·Lucia PiacentiniSergio Pimpinelli
Nov 15, 2003·Science·Jernej UleRobert B Darnell
May 21, 2005·Nucleic Acids Research·Filomena De LuciaFang-Lin Sun
Jul 1, 2006·Science·Vasily V VaginPhillip D Zamore
Feb 20, 2007·Current Biology : CB·Z Jin, Ting Xie
Feb 27, 2007·Current Biology : CB·Joseph K ParkQinghua Liu
Mar 9, 2007·Development·Jean Z MainesDennis M McKearin
Apr 21, 2007·Science·Margaret T Fuller, Allan Spradling
May 18, 2007·Annual Review of Cell and Developmental Biology·Judith Kimble, Sarah L Crittenden
Nov 13, 2007·Development·Lele YangDahua Chen
Oct 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Cindy Y Jao, Adrian Salic
Nov 22, 2008·Cold Spring Harbor Symposia on Quantitative Biology·T XieN Zhang
Mar 25, 2009·Developmental Biology·Andrew M EpsteinDaniela C Zarnescu
Feb 18, 2010·Genes & Development·Jerome S MenetMichael Rosbash
Apr 29, 2010·Epigenetics & Chromatin·Jeremy P BrownPrim B Singh
Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Susan EliazerMichael Buszczak
Jul 28, 2011·Cold Spring Harbor Perspectives in Biology·Allan SpradlingShosei Yoshida
Jan 21, 2014·Methods : a Companion to Methods in Enzymology·Andrew M Hudson, Lynn Cooley
Mar 1, 2014·Developmental Cell·Dong YanNorbert Perrimon
Jul 17, 2015·F1000Research·Gyanesh Singh
Sep 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Dong Yan, Norbert Perrimon
Oct 28, 2015·The Journal of Cell Biology·Jun Wei PekRuther Teo Zheng
Dec 3, 2015·Scientific Reports·Yalan Xing, Willis X Li
Aug 16, 2016·International Journal of Cell Biology·Ranjeet Singh Mahla

Methods Mentioned

BETA
transgenic
PCR
CLIP
immunoprecipitation
ChIP
electrophoresis

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