Mar 29, 2020

Left-handed DNA-PAINT for improved superresolution imaging in the nucleus.

BioRxiv : the Preprint Server for Biology
H. GeertsemaHelge Ewers


DNA point accumulation in nanoscale topography (DNA-PAINT) advances super-resolution microscopy with superior resolution and multiplexing capabilities. However, cellular DNA may interfere with this single-molecule localization technique based on DNA-DNA hybridization. Here, we introduce left-handed DNA (L-DNA) oligomers that do not hybridize to naturally present R-DNA and demonstrate that L-DNA PAINT has the same specificity and multiplexing capability as R-DNA PAINT, but greatly improves specific visualization of nuclear target molecules.

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Mentioned in this Paper

Meiotic Cell Cycle
Benign Neoplasm of Testis
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Histology Procedure
Neoplasm of Uncertain or Unknown Behavior of Testis
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