Heterocomplex formation by Arp4 and β-actin is involved in the integrity of the Brg1 chromatin remodeling complex

Journal of Cell Science
Naoki NishimotoMasatoshi Fujita

Abstract

Although nuclear actin and Arps (actin-related proteins) are often identified as components of multi-protein chromatin-modifying enzyme complexes, such as chromatin remodeling and histone acetyltransferase (HAT) complexes, their molecular functions still remain largely elusive. Here, we investigated the role of human Arp4 (BAF53, also known as actin-like protein 6A) in Brg1-containing chromatin remodeling complexes. Depletion of Arp4 by RNA interference impaired the integrity of these complexes and accelerated the degradation of Brg1, indicating a crucial role in their maintenance, at least in certain human cell lines. We further found that Arp4 can form a heterocomplex with β-actin. Based on structural similarities between conventional actin and Arp4, and the assumption that actin-Arp4 binding might mimic actin-actin binding, we introduced a series of mutations in Arp4 that might be expected to impair its interaction with β-actin. Some of them indeed caused reduced binding to β-actin. Interestingly, such mutant Arp4 proteins also showed reduced incorporation into Brg1 complexes, and their interaction with Myc-associated complexes as well as Tip60 HAT complexes were also impaired. Based on these findings, we propose that β-acti...Continue Reading

References

Oct 1, 1992·Genetics·K F WertmanD Botstein
Oct 30, 1992·Science·K F Wertman, D G Drubin
Sep 6, 1990·Nature·W KabschK C Holmes
Feb 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W Kabsch, K C Holmes
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R D MullinsT D Pollard
Sep 3, 1998·The Journal of Biological Chemistry·C L PetersonB T Chait
Mar 17, 1999·Molecular Cell·M L PhelanR E Kingston
Jul 28, 2001·Science·E M De La Cruz, T D Pollard
Nov 27, 2001·Science·R C RobinsonT D Pollard
Mar 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Oliver J RandoGerald R Crabtree
Jun 5, 2002·Annual Review of Biochemistry·Ivan A OlaveGerald R Crabtree
Oct 9, 2002·Genes & Development·Ivan OlaveGerald R Crabtree
Nov 20, 2002·Biochemical and Biophysical Research Communications·Yukiko KurodaMasahiko Harata
Feb 26, 2004·Experimental Cell Research·Nathalie Fomproix, Piergiorgio Percipalle
May 4, 2004·Molecular and Cellular Biology·Kairong CuiKeji Zhao
May 4, 2004·Nature Reviews. Molecular Cell Biology·Blaine T BettingerDavid C Amberg
Aug 17, 2004·Trends in Cell Biology·Carolyn A BlessingHolly V Goodson
Oct 27, 2004·Nature Cell Biology·Wilma A HofmannPrimal de Lanerolle
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Brad J NolenThomas D Pollard
Nov 24, 2004·Nature Cell Biology·Vlada V PhilimonenkoIngrid Grummt
Dec 3, 2004·Genes & Development·Ping HuNouria Hernandez
Feb 16, 2005·Nature Structural & Molecular Biology·Alexander KukalevPiergiorgio Percipalle
Oct 1, 2005·Molecular Biology of the Cell·Jean MullerBarbara Winsor
Jul 13, 2006·Journal of Cell Science·Yasutoshi TatsumiMasatoshi Fujita
Jul 27, 2007·Molecular Biology of the Cell·Kiwon LeeHyockman Kwon
Dec 29, 2007·Molecular Biology of the Cell·Nozomi SugimotoMasatoshi Fujita
Apr 15, 2008·Nature Structural & Molecular Biology·Heather SzerlongBradley R Cairns

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Citations

Jan 1, 2013·Cellular and Molecular Life Sciences : CMLS·Kei Miyamoto, J B Gurdon
Apr 5, 2013·Nature Structural & Molecular Biology·Blaine Bartholomew
Aug 5, 2014·Trends in Cell Biology·Christian B Gerhold, Susan M Gasser
Feb 6, 2016·Histochemistry and Cell Biology·Ildikó KristóPéter Vilmos
Oct 31, 2014·Bioscience, Biotechnology, and Biochemistry·Shota YamazakiMasahiko Harata
Jul 17, 2016·Biological Psychiatry·Zi-Jun WangDavid M Dietz
Jul 19, 2017·Scientific Reports·Victor V TatarskiyNataliya V Soshnikova
Mar 23, 2017·Frontiers in Genetics·Shinji MisuKei Miyamoto
Oct 16, 2018·Frontiers in Genetics·Naeh L Klages-MundtXuetong Shen
Dec 23, 2020·Journal of Biochemistry·Tomas VenitPiergiorgio Percipalle
Jan 23, 2021·Journal of Biochemistry·Kei Miyamoto, Masahiko Harata
Jan 8, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yang XiaoWeei-Chin Lin
Dec 6, 2017·Biochemical and Biophysical Research Communications·Xin Xie, Piergiorgio Percipalle

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