Heterodimerization of the epidermal-growth-factor (EGF) receptor and ErbB2 and the affinity of EGF binding are regulated by different mechanisms

The Biochemical Journal
L E JohannessenI H Madshus

Abstract

When clathrin-dependent endocytosis is inhibited in HeLa cells by overexpression of a K44A (Lys(44)-->Ala) mutant of the GTPase dynamin, high-affinity binding of epidermal growth factor (EGF) to the EGF receptor (EGFR) is disrupted [Ringerike, Stang, Johannessen, Sandnes, Levy and Madshus (1998) J. Biol. Chem. 273, 16639-16642]. We now report that the effect of [K44A]dynamin on EGF binding was counteracted by incubation with the non-specific kinase inhibitor staurosporine (SSP), implying that a protein kinase is responsible for disrupted high-affinity binding of EGF upon overexpression of [K44A]dynamin. The effect of [K44A]dynamin on EGF binding was not due to altered phosphorylation of the EGFR, suggesting that the activated kinase is responsible for phosphorylation of a substrate other than EGFR. The number of EGFR molecules was increased in cells overexpressing [K44A]dynamin, while the number of proto-oncoprotein ErbB2 molecules was unaltered. EGF-induced receptor dimerization was not influenced by overexpression of [K44A]dynamin. ErbB2-EGFR heterodimer formation was found to be ligand-independent, and the number of heterodimers was not altered by overexpression of [K44A]dynamin. Neither SSP nor the phorbol ester PMA, which ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 1, 1992·Neuron·J Schlessinger, A Ullrich
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·X L QuianM I Greene
Mar 1, 1992·Experimental Cell Research·P M van Bergen en HenegouwenJ Boonstra
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B A FriedmanM R Rosner
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M Böni-Schnetzler, P F Pilch
Oct 4, 1984·Nature·J DownwardM D Waterfield
Feb 1, 1984·Molecular and Cellular Endocrinology·W R RackoffH S Earp
Nov 1, 1994·The Journal of Cell Biology·H DamkeS L Schmid
Jun 30, 1997·FEBS Letters·M A Van der HeydenP M Van Bergen en Henegouwen
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·L M WangJ H Pierce
May 13, 1999·The Journal of Biological Chemistry·A J BarbierT B Patel

❮ Previous
Next ❯

Citations

Feb 14, 2003·Current Oncology Reports·Rebecca G Pomerantz, Jennifer Rubin Grandis
Mar 22, 2003·Experimental Cell Research·Robert N JorissenAntony W Burgess
May 22, 2003·Trends in Plant Science·Gabrielle TichtinskyThierry Gaude
Jul 10, 2004·Human Gene Therapy·Michele W L TengPhillip K Darcy
Aug 8, 2006·Biochimica Et Biophysica Acta·Sinto SebastianAngelo Paradiso
Nov 20, 2002·Molecular & Cellular Proteomics : MCP·Christopher D KrauseSidney Pestka
Dec 7, 2007·The Journal of Biological Chemistry·Katerina SideraEvangelia Patsavoudi
Feb 12, 2009·Molecular Cancer Research : MCR·Nina Marie PedersenInger Helene Madshus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.