Heteroduplex PCR analysis of rearranged T cell receptor genes for clonality assessment in suspect T cell proliferations

Leukemia
A W LangerakJ J van Dongen

Abstract

Molecular analysis of T cell receptor (TCR) genes is frequently used to prove or exclude clonality and thereby support the diagnosis of suspect T cell proliferations. PCR techniques are more and more being used for molecular clonality studies. The main disadvantage of the PCR-based detection of clonal TCR gene rearrangements, is the risk of false-positive results due to 'background' amplification of similar rearrangements in polyclonal reactive T lymphocytes. Therefore, PCR-based clonality assessment should include analyses that discern between PCR products derived from monoclonal and polyclonal cell populations. One such method is heteroduplex analysis, in which homo- and heteroduplexes resulting from denaturation (at 94 degrees C) and renaturation (at lower temperatures) of PCR products, are separated in non-denaturing polyacrylamide gels based on their conformation. After denaturation/renaturation, PCR products of clonally rearranged TCR genes give rise to homoduplexes, whereas in case of polyclonal cells heteroduplexes with heterogeneous junctions are formed. We studied heteroduplex PCR analysis of TCR gene rearrangements with respect to the time and temperature of renaturation and the size of the PCR products. Variation in...Continue Reading

Citations

Aug 5, 2000·Cytometry·R van den BeemdJ J van Dongen
Jan 8, 2003·Pathology Oncology Research : POR·Samina NooraliShahid Pervez
Mar 14, 2007·Digestive Diseases and Sciences·Boris SapoznikovYaron Niv
Feb 22, 2005·Cancer Genetics and Cytogenetics·Elaine LeungMohamed Abdelhaleem
Apr 27, 2007·Leukemia·J ZunaJ Trka
Sep 5, 2001·The British Journal of Dermatology·M W BekkenkR Willemze
Jun 19, 2001·British Journal of Haematology·M BieringsJ J van Dongen
Mar 27, 2003·The American Journal of Surgical Pathology·Anke van den BergSibrand Poppema
Oct 17, 2006·Pathology International·Rocío HassanIlana R Zalcberg
Dec 25, 2002·Journal of Clinical Pathology·E HodgesJ L Smith
Jun 1, 2001·Journal of Neurology, Neurosurgery, and Psychiatry·D GestriL Massacesi
Oct 8, 2005·The Journal of Clinical Investigation·Stephan EhlPaul Fisch
Jan 17, 2009·Blood·Charlotte V CoxAllison Blair
Jun 24, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Elaine Coustan-SmithDario Campana
Jun 17, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Tomasz SzczepańskiJacques J M van Dongen
Aug 12, 2009·Journal of Hematopathology·Patricia J T A GroenenJohan H J M van Krieken
Apr 12, 2011·European Journal of Pediatrics·Mirjam van der Burg, Andy R Gennery
Sep 8, 2000·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·N T BeaubierD S Viswanatha
Sep 17, 2013·World Journal of Gastroenterology : WJG·Guo-Dong ShanGuo-Qiang Xu
Sep 10, 2003·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Carlos A ScrideliLuiz G Tone
Dec 1, 2007·Expert Opinion on Medical Diagnostics·Anton W LangerakJacques Jm van Dongen
Feb 8, 2011·Clinics in Laboratory Medicine·Dorothee Bienzle, William Vernau

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