Heterogeneities in Nanog Expression Drive Stable Commitment to Pluripotency in the Mouse Blastocyst

Cell Reports
Panagiotis XenopoulosAnna-Katerina Hadjantonakis

Abstract

The pluripotent epiblast (EPI) is the founder tissue of almost all somatic cells. EPI and primitive endoderm (PrE) progenitors arise from the inner cell mass (ICM) of the blastocyst-stage embryo. The EPI lineage is distinctly identified by its expression of pluripotency-associated factors. Many of these factors have been reported to exhibit dynamic fluctuations of expression in embryonic stem cell cultures. Whether these fluctuations correlating with ICM fate choice occur in vivo remains an open question. Using single-cell resolution quantitative imaging of a Nanog transcriptional reporter, we noted an irreversible commitment to EPI/PrE lineages in vivo. A period of apoptosis occurred concomitantly with ICM cell-fate choice, followed by a burst of EPI-specific cell proliferation. Transitions were occasionally observed from PrE-to-EPI, but not vice versa, suggesting that they might be regulated and not stochastic. We propose that the rapid timescale of early mammalian embryonic development prevents fluctuations in cell fate.

References

Dec 8, 2004·Mechanisms of Development·Shin-Ya HatanoTakashi Tada
Nov 6, 2007·Development·Jens-Erik Dietrich, Takashi Hiiragi
May 4, 2010·Developmental Biology·Daniel M Messerschmidt, Rolf Kemler
Dec 17, 2011·Developmental Cell·Stephen FrankenbergClaire Chazaud
Aug 3, 2012·Cold Spring Harbor Perspectives in Biology·Jennifer Nichols, Austin Smith
Oct 30, 2012·Nature Cell Biology·Ben D MacArthurIhor R Lemischka
Jan 26, 2013·Genesis : the Journal of Genetics and Development·Nadine SchrodeAnna-Katerina Hadjantonakis
Jun 12, 2013·Cell Reports·Sophie M MorganiJoshua M Brickman
Jul 6, 2013·Cell Stem Cell·Austin Smith
Jul 6, 2013·Cell Stem Cell·Adam FilipczykTimm Schroeder
Mar 1, 2014·Developmental Cell·Mélanie A Eckersley-MaslinDavid L Spector
Mar 1, 2014·Developmental Cell·Anne-Valerie GendrelEdith Heard
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Frederick E Tan, Michael B Elowitz
May 20, 2014·Developmental Cell·Nadine SchrodeAnna-Katerina Hadjantonakis
May 29, 2014·Development·Maria-Elena Torres-Padilla, Ian Chambers

❮ Previous
Next ❯

Citations

Feb 4, 2016·Biophysical Journal·Laurane De MotGeneviève Dupont
Dec 15, 2015·Nature Methods·Petr StrnadJan Ellenberg
Dec 15, 2015·Development, Growth & Differentiation·Kouji Komatsu, Toshihiko Fujimori
Jul 18, 2015·Seminars in Cell & Developmental Biology·Néstor SaizAnna-Katerina Hadjantonakis
Sep 1, 2015·Seminars in Cell & Developmental Biology·Silvia Muñoz-DescalzoAlfonso Martinez Arias
Dec 15, 2015·Stem Cell Reports·Ronan RussellAlexander Kleger
Jan 16, 2016·Stem Cells International·Clair E WeidgangMartin Mueller
Sep 5, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Narumol Bhummaphan, Pithi Chanvorachote
Aug 25, 2016·Nature·Tomonori NakamuraMitinori Saitou
Oct 11, 2016·ELife·Robert S IllingworthJoshua M Brickman
Nov 20, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sergio MencheroMiguel Manzanares
Oct 5, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Steven Blinka, Sridhar Rao
Apr 7, 2016·Development·Claire Chazaud, Yojiro Yamanaka
Aug 11, 2018·Annual Review of Cell and Developmental Biology·Hui Ting Zhang, Takashi Hiiragi
Apr 9, 2019·ELife·Sergio MencheroMiguel Manzanares
May 9, 2019·Development·Julia DeathridgeJonathan R Chubb
Mar 1, 2019·The EMBO Journal·Julio Sainz de AjaMiguel Manzanares
Sep 13, 2019·Genesis : the Journal of Genetics and Development·Maiko TeradaAtsushi Suzuki
May 16, 2020·PloS One·Sabine C FischerSilvia Muñoz-Descalzo
Jul 19, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Jonathan R Chubb
Jul 24, 2020·Development·Berenika Płusa, Anna Piliszek
May 1, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Claire S SimonChristian Schröter
Aug 28, 2020·Development·Alba Redó Riveiro, Joshua Mark Brickman
Jun 15, 2017·BMC Developmental Biology·Sophie MorganiAnna-Katerina Hadjantonakis
Jul 13, 2017·PLoS Biology·Silas Boye NissenAla Trusina
Dec 12, 2018·Biology Open·Claire S SimonAnna-Katerina Hadjantonakis
Apr 19, 2018·Nature Reviews. Molecular Cell Biology·Blanca Pijuan-SalaBerthold Göttgens
Jun 27, 2017·NPJ Systems Biology and Applications·Alen TosenbergerGeneviève Dupont
Sep 2, 2020·Annual Review of Genetics·Jonathan FiorentinoAntonio Scialdone
Feb 15, 2021·Development, Growth & Differentiation·Yayoi Toyooka
Dec 12, 2020·Cell·Meng Zhu, Magdalena Zernicka-Goetz
Apr 13, 2021·Frontiers in Cell and Developmental Biology·Puja Agrawal, Sridhar Rao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.