Heterogeneity among 16S-23S rRNA intergenic spacers of species within the 'Streptococcus milleri group'.

Microbiology
R A WhileyL M C Hall

Abstract

The 16S-23S rRNA intergenic spacer has been suggested as a suitable region of the bacterial genome from which to derive useful taxonomic information, particularly with regard to identification at the species level. To investigate this approach as an aid to the identification of the three species comprising the 'Streptococcus milleri group' (SMG), the spacers of isolates of Streptococcus intermedius, Streptococcus anginosus and Streptococcus constellatus were amplified by PCR and length polymorphisms determined by agarose gel electrophoresis. Phenotypically atypical isolates which had been identified presumptively as belonging to these three species were also included. Spacers from two representatives of each spacer length found within the three SMG species were sequenced. 16S-23S rRNA intergenic spacer length polymorphisms allowed discrimination between S. anginosus (350 bp or 450 bp amplification product) and S. constellatus (380 bp amplification product), species that are difficult to differentiate phenotypically. S. intermedius (330 bp or 450 bp amplification product) and S. anginosus (350 bp or 450 bp amplification product) were not reliably distinguished by this method but are phenotypically distinct. Sequencing data demon...Continue Reading

References

Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E A MorganM Nomura
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R A Young, J A Steitz
Aug 1, 1992·Journal of Clinical Microbiology·J R KostmanT L Stull
Oct 1, 1991·International Journal of Systematic Bacteriology·R W BentleyM D Collins
Aug 1, 1991·PCR Methods and Applications·T BarryF Gannon
Jun 1, 1987·Microbiological Reviews·C R Woese
Mar 1, 1988·Reviews of Infectious Diseases·J Gossling
May 1, 1982·Journal of Clinical Microbiology·K L Ruoff, L J Kunz
Feb 1, 1994·The Journal of Infectious Diseases·R Frothingham, K H Wilson
Feb 1, 1993·Molecular and Cellular Probes·G L McLaughlinR B Carey

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Citations

Dec 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L M HallG Urwin
Sep 3, 2003·FEMS Microbiology Letters·Andrés CasanovaMatilde Jashés M
Mar 27, 1999·Oral Microbiology and Immunology·R A Whiley, D Beighton
Mar 12, 1998·Journal of Clinical Periodontology·A P ColomboS S Socransky
Sep 15, 2001·Antimicrobial Agents and Chemotherapy·A P RobertsP Mullany
Apr 17, 2001·Antimicrobial Agents and Chemotherapy·M TracyD M Musher
Oct 27, 2006·Journal of Clinical Microbiology·Sheng Kai TungTsung Chain Chang
Jan 13, 2004·Journal of Clinical Microbiology·William HurtleDavid Norwood
Apr 9, 2004·Journal of Clinical Microbiology·Wen-Chu ChiangPo-Ren Hsueh
Apr 9, 2005·Journal of Clinical Microbiology·Chao Chien ChenTsung Chain Chang
Jan 13, 2005·Canadian Journal of Microbiology·Douglas W Dingman
Jan 28, 2009·BMC Biology·Cynthia J BishopBrian G Spratt
Jul 10, 2001·Oral Microbiology and Immunology·Y P PanP W Caufield
Sep 9, 2006·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Yu-Min ChuangPeilin Lee
Jul 25, 2017·Tropical Doctor·Priya DattaAshok K Attri
Jun 10, 2004·Journal of Applied Microbiology·S Pérez-LuzV Catalán
Feb 3, 2005·Environmental Microbiology·Gabriel ZwartHerman J Gons
Mar 1, 1997·Microbiology·Afaf NaïmiChristiane Branlant

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