Heterogeneity and neurovascular integration of intraportally transplanted islets revealed by 3-D mouse liver histology.

American Journal of Physiology. Endocrinology and Metabolism
Chien-Chia ChenShiue-Cheng Tang

Abstract

Intraportal islet transplantation has been clinically applied for treatment of unstable type 1 diabetes. However, in the liver, systematic assessment of the dispersed islet grafts and the graft-hepatic integration remains difficult, even in animal models. This is due to the lack of global and in-depth analyses of the transplanted islets and their microenvironment. Here, we apply three-dimensional (3-D) mouse liver histology to investigate the islet graft microstructure, vasculature, and innervation. Streptozotocin-induced diabetic mice were used in syngeneic intraportal islet transplantation to achieve euglycemia. Optically cleared livers were prepared to enable 3-D morphological and quantitative analyses of the engrafted islets. 3-D image data reveal the clot- and plaque-like islet grafts in the liver: the former are derived from islet emboli and associated with ischemia, whereas the latter (minority) resemble the plaques on the walls of portal vessels (e.g., at the bifurcation) with mild, if any, perigraft tissue damage. Three weeks after transplantation, both types of grafts are revascularized, yet significantly more lymphatics are associated with the plaque- than clot-like grafts. Regarding the islet reinnervation, both typ...Continue Reading

References

Feb 11, 1986·The American Journal of Physiology·B AhrénG J Taborsky
May 16, 1986·Science·F C Schuit, D G Pipeleers
May 1, 1997·The American Journal of Physiology·B E DunningA D Cherrington
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·T RosenbaumM Hiriart
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T A PartanenK Alitalo
Dec 6, 2002·The Journal of Clinical Endocrinology and Metabolism·Boaz HirshbergDavid M Harlan
Jan 8, 2004·Growth Factors·Román VidaltamayoMarcia Hiriart
Apr 26, 2006·Clinical and Experimental Immunology·T LinnD Brandhorst
Feb 26, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C TosoT Berney
Dec 10, 2009·Transplantation·Christian TosoA M James Shapiro
Jan 5, 2011·Transplantation·Frantisek SaudekMilan Hájek
Sep 8, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Y-A LiuS-C Tang
Mar 17, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuan-An LiuShiue-Cheng Tang
Feb 13, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Y D MullerT Berney
Jan 16, 2014·American Journal of Physiology. Endocrinology and Metabolism·Jyuhn-Huarng JuangShiue-Cheng Tang
Sep 10, 2014·Diabetes, Obesity & Metabolism·S-C TangH-J Chien
Sep 10, 2014·Diabetes, Obesity & Metabolism·G J TaborskyT O Mundinger
Oct 27, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·H-J ChienS-C Tang
May 22, 2016·Diabetes Care·Bernhard J HeringUNKNOWN Clinical Islet Transplantation Consortium
Nov 12, 2016·Nature Reviews. Endocrinology·A M James ShapiroCamillo Ricordi
Aug 31, 2017·Diabetologia·Shiue-Cheng TangMichael S German
Sep 3, 2017·Diabetologia·Shiue-Cheng TangPankaj J Pasricha
Feb 2, 2018·Endocrinology·Jonas L FowlerManami Hara
Feb 3, 2018·Islets·Anissa GambleA M James Shapiro
Feb 8, 2018·Peptides·Gina L C Yosten
Feb 15, 2018·Journal of Visualized Experiments : JoVE·Elizabeth ButterworthMartha Campbell-Thompson
Mar 23, 2018·European Review for Medical and Pharmacological Sciences·B Antonioli, M Galuzzi
Dec 13, 2018·Endocrine Reviews·Michael R Rickels, R Paul Robertson
Sep 12, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hung-Jen ChienShiue-Cheng Tang
Sep 20, 2019·Lancet·Marie-Christine VantyghemMichael R Rickels
Oct 17, 2019·Diabetes Care·Marie-Christine VantyghemUNKNOWN Members of the Spanish Back Pain Research Network Task Force for the Improvement of Inter-Disciplinary Management of Spinal
Mar 22, 2020·Diabetes Care·Bart O Roep
Apr 2, 2020·Physiological Reports·Juan V Sanchez-AndresWilly J Malaisse
May 19, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Cambrian Y Liu, D Brent Polk

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