Heterogeneity and organization of the ribosomal RNA genes of Cucurbita maxima

Plant Molecular Biology
R J KellyA Siegel

Abstract

Thirty-six clones were recovered from Cucurbita maxima genomic DNA which had been enriched for rDNA and cleaved at the unique repeat unit Hind III site. Twenty-nine of these, which contain complete rDNA units, were compared to a standard whose intergenic spacer (IGS) nucleotide sequence has been determined. Twenty-one are identical in length and restriction site pattern. Eight which differ from the standard in length do so because of addition or deletion of varying numbers of IGS subrepetitive units of two different classes, with four of the length variants being different in both of these classes. Seven clones were isolated which contain incomplete repeat units, six of which are composites of rDNA and non-rDNA material. They have been cleaved at the unique rDNA Hind III site at one end and at a non-rDNA Hind III site at the other. We consider it most likely that these are derived from the termini of repeat unit tandem arrays, although other explanations are possible. Twelve individual plants of two different cultivars were examined for heterogeneity of IGS length distribution. They all appear to be identical in this regard.

References

Jan 1, 1986·Annual Review of Biochemistry·B Sollner-Webb, J Tower
Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·K Matsuda, A Siegel
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M A Saghai-MaroofR W Allard
Jan 1, 1980·Annual Review of Biochemistry·E O Long, I B Dawid
May 1, 1983·Plant Physiology·A Siegel, K Kolacz
Oct 1, 1972·Genetics·R B GoldbergA Siegel
Jun 1, 1986·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N O PolansW F Thompson
Dec 1, 1982·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R Appels, J Dvořák
Sep 1, 1987·Plant Molecular Biology·S O Rogers, A J Bendich
Mar 1, 1985·Plant Molecular Biology·S O Rogers, A J Bendich
Sep 1, 1986·Plant Molecular Biology·S O RogersA J Bendich
Mar 1, 1987·Evolution; International Journal of Organic Evolution·Gerald H Learn, Barbara A Schaal

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