Heterogeneity and pigment composition of isolated photosystem II reaction centers

Biochemistry
D ZhelevaJ Barber

Abstract

Photosystem II reaction centers (RC) isolated from peas (Pisum sativum L) purified by ionexchange chromatography were shown, by high-performance liquid chromatography (HPLC) size-exclusion analyses, to consist of a mixture of monomers (180 +/- 20 kDa) and dimers (390 +/- 35 kDa). Both fractions were resolved and purified by sucrose density gradient centrifugation and their homogeneity was demonstrated in size-exclusion HPLC elution profiles. Also present in the nonresolved preparation and the monomeric fraction were low levels of CP47 apoprotein (1.8% and 0.9% apoprotein of that found in a CP47-RC preparation). This CP47 contamination could maximally account for 0.41 and 0.22 Chl/RC, respectively, based on 22 chlorophylls being bound to each CP47 protein. The level of CP47 apoprotein was undetectable in the dimeric fractions. Pigment analysis using reverse-phase HPLC confirmed that contamination by chlorophyll bound to the CP47 apoprotein in the nonresolved RC preparation was low and that the ratio of chlorophyll a to pheophytin a remained 6 when the preparation was separated into its monomeric and dimeric components. We conclude, in agreement with earlier work, that the reaction center of PSII, when isolated using mild deterge...Continue Reading

Citations

Aug 10, 2001·Trends in Plant Science·P Albertsson
Aug 27, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Elmars KrauszSindra Peterson Arsköld
Jan 23, 1999·European Journal of Biochemistry·G TsiotisD Ghanotakis
Jul 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Thomas S BibbyJames Barber
May 23, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·I YruelaR Picorel
Jun 20, 1998·The Journal of Biological Chemistry·D ZhelevaJ Barber
Feb 7, 2015·Molecular Biology and Evolution·Tanai CardonaA William Rutherford
Mar 11, 2020·Journal of Molecular Modeling·Natalia KulikDavid Řeha

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